{"product_id":"abcam-ab40763","title":"Abcam, ab40763, Anti-CD45 antibody [EP322Y]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-CD45 antibody [EP322Y] (ab40763) is a rabbit monoclonal antibody detecting CD45 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human . - Biophysical QC for unrivalled batch-batch consistency - Over 50 publications - Trusted since 2006\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP322Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WB, ICC\/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:This antibody recognizes cytoplasmic domain of CD45.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-CD45 antibody [EP322Y] (ab40763) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC-P and WB.\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-CD45 antibody [EP322Y] (ab40763) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nAnti-CD45 antibody [EP322Y] (ab40763) has 13 independent reviews from customers.\u003cbr\u003e\nAnti-CD45 antibody [EP322Y] (ab40763) specifically detects CD45 (UniProt ID: P08575; Molecular weight: 145kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone EP322Y -\u003cbr\u003e\nab214437\u003cbr\u003e\nAntibody clone EP322Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor\u003cbr\u003e\n488, Alexa Fluor\u003cbr\u003e\n647, PE, PerCP, APC, Alexa Fluor\u003cbr\u003e\nab200315\u003cbr\u003e\nab200317\u003cbr\u003e\nab214501\u003cbr\u003e\nab220298\u003cbr\u003e\nab221245\u003cbr\u003e\nab303598\u003cbr\u003e\nCD45 regulates immune cell signaling and is crucial in the development and function of immune cells, with alterations linked to various immunodeficiencies and autoimmune diseases.\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nMouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.\u003cbr\u003e\nPathways\u003cbr\u003e\nScientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.\u003cbr\u003e\nScientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844045459625,"sku":"ab40763","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab40763","provider":"Iright","version":"1.0","type":"link"}