{"product_id":"abcam-ab40772","title":"Abcam, ab40772, Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) is a rabbit monoclonal antibody detecting E Cadherin in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF, mIHC . Suitable for  Human . - KO validated for confirmed specificity - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 750 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EP700Y,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, Flow Cyt (Intra), Flow Cyt, IHC-P, WB, mIHCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. This antibody has been tested on human samples in both WB and IHC. Customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAbcam recommended secondaries - Goat Anti-Rabbit HRP (\u003cbr\u003e\nab205718\u003cbr\u003e\n) and Goat Anti-Rabbit Alexa Fluor® 488 (\u003cbr\u003e\nab150077\u003cbr\u003e\n). Or search our wide range of secondary antibodies for use with your experiment.\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, Flow Cyt (Intra), ICC\/IF, IHC-P, WB, mIHC in human samples.\u003cbr\u003e\nAnti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) specifically detects E Cadherin (UniProt ID: P12830; Molecular weight: 80kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality manufacturing and validation processes ensure Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.\u003cbr\u003e\nThe specificity of Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has been confirmed by testing in knockout samples.\u003cbr\u003e\nAnti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has been cited over 750 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has 21 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nAntibody clone EP700Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC (\u003cbr\u003e\nab185013\u003cbr\u003e\nab194982\u003cbr\u003e\nab206878\u003cbr\u003e\nab206880\u003cbr\u003e\nab224959\u003cbr\u003e\nab224960\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nSpecies reactivity\u003cbr\u003e\nRat: We have preliminary internal testing data to indicate this antibody may not react with this species.\u003cbr\u003e\nPlease\u003cbr\u003e\ncontact us\u003cbr\u003e\nfor more information.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nE-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nE-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.\u003cbr\u003e\nPathways\u003cbr\u003e\nE-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.\u003cbr\u003e\nE-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844027404457,"sku":"ab40772","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab40772","provider":"Iright","version":"1.0","type":"link"}