{"product_id":"abcam-ab46087","title":"Abcam, ab46087, Human TNF alpha ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 2 x 96Tests\u003cbr\u003e\nHuman TNF alpha ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TNF alpha in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 8 pg\/mL,\u003cbr\u003e\nRange:25 - 800 pg\/mL,\u003cbr\u003e\nAssay time:3h 45m,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman TNF alpha ELISA kit (ab46087) is a quantitative ELISA assay for the measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.\u003cbr\u003e\nA monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.\u003cbr\u003e\nThis kit will recognize both endogenous and recombinant Human TNF alpha.\u003cbr\u003e\nGet results in 90 minutes with Human TNF alpha ELISA Kit (\u003cbr\u003e\nab181421\u003cbr\u003e\n) from our SimpleStep ELISA\u003cbr\u003e\nrange.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843543322793,"sku":"ab46087","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab46087","provider":"Iright","version":"1.0","type":"link"}