{"product_id":"abcam-ab46917","title":"Abcam, ab46917, Anti-CD28 antibody [B-T3] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 200µg\u003cbr\u003e\nMouse Monoclonal CD28 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD28 protein.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:B-T3,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Cell preparation containing CD28 protein. The exact immunogen used to generate this antibody is proprietary information.P10747,\u003cbr\u003e\nSpecificity:Recognises the B7\/BB1 receptor (B7\/BB1 R), a 44 kDa protein.Inhibits mixed lymphocyte culture. Co-stimulates T-cell proliferation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Ion exchange chromatography, Purification notes-Sterile-filtered through 0.22 µm and treated to remove endotoxins. Carrier and preservative free., Storage buffer-pH: 7.3 - 7.5Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, -20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD28 is integral to the PI3K\/Akt and MAPK\/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.\u003cbr\u003e\nCD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855381581993,"sku":"ab46917","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab46917","provider":"Iright","version":"1.0","type":"link"}