{"product_id":"abcam-ab4832","title":"Abcam, ab4832, Anti-Paxillin (phospho Y31) antibody","description":"\u003cp\u003eSize: 50µL\u003cbr\u003e\nRabbit Polyclonal Paxillin phospho Y31 antibody. Suitable for WB, ICC\/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human Paxillin phospho Y31.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human Paxillin phospho Y31. The exact immunogen used to generate this antibody is proprietary information.P49023\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 31., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPaxillin also known as PXN is a cytoskeletal protein involved in the assembly of focal adhesions. It has a molecular weight of approximately 68 kDa. You will find Paxillin expressed widely in various tissues including the liver colon and lung. Paxillin plays a part in cell motility by interacting with other focal adhesion proteins. It plays a significant mechanical role by serving as a docking site for structural and signaling molecules.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPaxillin serves key functions in cellular processes such as migration proliferation and survival. It interacts with different proteins including vinculin and talin forming part of a complex at the focal adhesion sites. The phosphorylation state of Paxillin can modulate its interactions and in this way influence the assembly of signaling complexes that control dynamic cellular processes.\u003cbr\u003e\nPathways\u003cbr\u003e\nPaxillin participates in the integrin signaling and MAPK pathways. It operates by linking integrin receptors to the actin cytoskeleton facilitating signal transduction. Paxillin phosphorylation is an important regulatory mechanism within these pathways. In particular its interaction with focal adhesion kinase (FAK) and Src family kinases signifies its role in transmitting signals from the extracellular matrix to the cellular interior which impacts cell behavior and response.\u003cbr\u003e\nPaxillin has links with osteosarcoma and lung cancer. Deregulation of Paxillin expression or its phosphorylation state contributes to oncogenic transformation and tumor progression. In cancer Paxillin associates with altered activity of proteins such as Src and FAK exacerbating disease states. This relationship highlights Paxillin's potential as a biomarker and therapeutic target in treating related malignancies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848623935657,"sku":"ab4832","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab4832","provider":"Iright","version":"1.0","type":"link"}