{"product_id":"abcam-ab49032","title":"Abcam, ab49032, Recombinant Hepatitis C Virus NS5 protein","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRecombinant Hepatitis C Virus NS5 protein is a Hepatitis C virus (isolate EUH1480) Fragment protein, in the 2061 to 2302 aa range, expressed in Escherichia coli, with \u0026gt;95%, suitable for ELISA, WB, Flow Cyt.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:Flow Cyt, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:O39928,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Hepatitis C virus (isolate EUH1480),\u003cbr\u003e\nStorage buffer:pH: 7.2 - 7.6Constituents: 50% Glycerol (glycerin, glycerine), 9% Urea, 0.395% Tris HCl, 0.2% Triton X-100\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Hepatitis C Virus NS5 also known as Nonstructural Protein 5A (NS5A) is a component of the hepatitis C virus polyprotein. It has a mass of approximately 56-58 kDa. NS5A is localized within the membrane-associated replication complex in hepatocytes where the hepatitis C virus replicates. This protein plays an important role in viral replication and assembly acting primarily in concert with other nonstructural proteins of the virus.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nHepatitis C Virus NS5A interacts with host cell machinery to modulate viral replication and assembly processes. NS5A forms part of a complex with other viral proteins such as NS3 and NS4A which are essential for the replication of the viral RNA. It participates in interactions that adjust the lipid composition of the host cell membranes facilitating efficient replication of the viral genome. Moreover NS5A has multiple phosphorylation states which influence its activity and role in the virus life cycle.\u003cbr\u003e\nPathways\u003cbr\u003e\nHepatitis C Virus NS5A participates in viral replication and immune modulation pathways. NS5A contributes significantly to the replication of the viral genome by interacting with cellular kinases like protein kinase R (PKR) which the virus uses to evade the host's immune response. Furthermore NS5A's interaction with the PI3K\/Akt pathway can promote cell survival and proliferation impacting the host's cellular environment to benefit the virus. This protein's ability to affect such pathways makes it an important target for antiviral therapies.\u003cbr\u003e\nHepatitis C Virus NS5A is strongly linked to chronic hepatitis C infection which can lead to liver cirrhosis and hepatocellular carcinoma. The NS5A protein affects not only the replication of the virus but also modulates the host's immune response and cellular processes. For instance NS5A interacts with tumor suppressor proteins such as p53 altering cell cycle regulation and contributing to the oncogenic potential of chronic HCV infection. Consequently NS5A is a principal target in the design of direct-acting antiviral agents for treating hepatitis C infections.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843904032937,"sku":"ab49032","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab49032","provider":"Iright","version":"1.0","type":"link"}