{"product_id":"abcam-ab53232","title":"Abcam, ab53232, Anti-Nitrotyrosine antibody [11C2]","description":"\u003cp\u003eSize: 500µL\u003cbr\u003e\nMouse Monoclonal Nitrotyrosine antibody. Suitable for ICC, ELISA, WB and reacts with Chemical samples. Cited in 10 publications. Immunogen corresponding to Chemical \/ Small Molecule corresponding to Nitrotyrosine.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:11C2,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:ICC, ELISA, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nSpecificity:This antibody reacts with free and bound Nitrotyrosine.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-Tissue culture supernatant, Storage buffer-Preservative: 0.02% Sodium azideConstituents: Tissue culture supernatant, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nNitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nNitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.\u003cbr\u003e\nPathways\u003cbr\u003e\nProteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.\u003cbr\u003e\nNitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46860506529961,"sku":"ab53232","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab53232","provider":"Iright","version":"1.0","type":"link"}