{"product_id":"abcam-ab5796","title":"Abcam, ab5796, Anti-PKC gamma (phospho T655) antibody","description":"\u003cp\u003eSize: 50µL\u003cbr\u003e\nRabbit Polyclonal KPCG phospho T655 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PRKCG phospho T655.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human, Mouse,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human PRKCG phospho T655. The exact immunogen used to generate this antibody is proprietary information.P05129,\u003cbr\u003e\nSpecificity:PKC alpha (69%) and beta 2 (62%) may cross-react in cells expressing high levels of these proteins.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine\/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC gamma. The final product is generated by affinity chromatography using a PKC gamma-derived peptide that is phosphorylated at threonine 655., Storage buffer-pH: 7.3Preservative: 0.05% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nProtein kinase C gamma (PKC gamma) also known as PKCγ is a member of the protein kinase C (PKC) family. This protein has a molecular weight of approximately 78 kDa. Expressed mainly in the central nervous system PKC gamma participates in various signaling pathways. It exists as a serine\/threonine kinase and plays a fundamental role in modulating cellular functions in neurons.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPKC gamma acts in the regulation of neuronal signaling processes and is a component of the synaptic transmission machinery. It associates with other proteins to form complex structures that influence physiological processes. PKC gamma contributes significantly to the functions of the central nervous system by modulating synaptic plasticity which is essential for learning and memory.\u003cbr\u003e\nPathways\u003cbr\u003e\nPKC gamma participates in the MAPK and PI3K signaling pathways which are critical in cell growth and survival. The protein interacts with other PKC isozymes and signaling molecules within these pathways playing a role in phosphorylating specific target proteins. These interactions facilitate the regulation of cell cycle progression and apoptosis indicating its importance in cellular homeostasis.\u003cbr\u003e\nAbnormal PKC gamma activity associates with spinocerebellar ataxia type 14 and addiction-related behavior. Spinocerebellar ataxia results from mutations affecting PKC gamma which disrupts its normal function and leads to neurodegeneration. Additionally PKC gamma’s interaction with other proteins such as GABAA receptors in addiction highlights potential therapeutic targets for treatment.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848598311081,"sku":"ab5796","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab5796","provider":"Iright","version":"1.0","type":"link"}