{"product_id":"abcam-ab6160","title":"Abcam, ab6160, Anti-Tubulin antibody [YL1\/2] - Loading Control","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-Tubulin antibody [YL1\/2] - Loading Control (ab6160) is a rat monoclonal antibody detecting Tubulin in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - Over 540 publications - Trusted since 2002\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rat,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:YL1\/2,\u003cbr\u003e\nIsotype:IgG2a,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Rat, Human, Mouse,\u003cbr\u003e\nApplications:IHC-P, WB, ICC\/IF, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nEpitope:The YL1\/2 monoclonal epitope has been mapped to the last 8 residues (GEEEGEEY) at the carboxy terminus of alpha tubulin when tyrosinated (PubMed IDs: 6415068, 6204858).\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct Specifications\u003cbr\u003e\nAnti-Tubulin antibody [YL1\/2] - Loading Control (ab6160) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC\/IF, IHC-P, WB in human, mouse, rat samples.\u003cbr\u003e\nTubulin antibodies are often used as loading controls in Western Blot. Anti-Tubulin antibody [YL1\/2] - Loading Control has been verified in Western Blot samples and detects a band at 50kDa Molecular weight.\u003cbr\u003e\nAnti-Tubulin antibody [YL1\/2] - Loading Control (ab6160) specifically detects Tubulin (UniProt ID: P68363; Molecular weight: 50kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nQuality and Validation\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-Tubulin antibody [YL1\/2] - Loading Control (ab6160) has high sensitivity and specificity.\u003cbr\u003e\nAnti-Tubulin antibody [YL1\/2] - Loading Control (ab6160) has been cited over 545 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAnti-Tubulin antibody [YL1\/2] - Loading Control (ab6160) has 36 independent reviews from customers.\u003cbr\u003e\nRelated Products\u003cbr\u003e\nConjugation-ready, carrier free format available for antibody clone YL1\/2 -\u003cbr\u003e\nab269872\u003cbr\u003e\nAntibody clone YL1\/2 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, HRP (\u003cbr\u003e\nab197737\u003cbr\u003e\nab197740\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 6.97% L-Arginine, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTubulin sometimes called DM1B is a globular protein essential for the formation of microtubules. It has a molecular mass of approximately 55 kDa. Tubulin is expressed in most eukaryotic cells and exists as two closely related subunits alpha-tubulin and beta-tubulin. These subunits polymerize to form linear microtubule structures. Anti-tubulin antibodies such as anti-tubulin Alexa 34 can detect these microtubule markers in cells allowing researchers to visualize cytoskeletal organization.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis core component of microtubule assembly is important for maintaining cell shape enabling intracellular transport and segregating chromosomes during cell division. As part of a complex tubulin interacts with various microtubule-associated proteins (MAPs) that regulate its dynamic assembly and disassembly. This regulation is important for processes like axonal transport in neurons and the movement of cilia and flagella in other cell types.\u003cbr\u003e\nPathways\u003cbr\u003e\nTubulin plays a significant role in the mitotic spindle assembly part of the cell cycle. This process is vital for the accurate segregation of chromosomes to daughter cells. Tubulin interacts with the kinesin and dynein motor proteins within this pathway which are essential for intracellular transport and mitosis. Another key pathway involving tubulin is the intracellular trafficking facilitated by motor proteins which is necessary for maintaining cell homeostasis and function.\u003cbr\u003e\nAberrant regulation or mutations in tubulin can lead to conditions such as cancer and neurodegenerative diseases. In cancer altered tubulin dynamics contribute to uncontrolled cell proliferation. Neurodegenerative disorders such as Alzheimer’s disease show involvement with tubulin through abnormal microtubule stability often linked to the MAP tau protein. Anti-tubulin antibodies including LAMP1 can help in research related to these conditions by offering methods to study tubulin distribution and function in diseased cells.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844158083241,"sku":"ab6160","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab6160","provider":"Iright","version":"1.0","type":"link"}