{"product_id":"abcam-ab6188","title":"Abcam, ab6188, Anti-M13 + Fd Bacteriophage Coat Proteins antibody","description":"\u003cp\u003eSize: 50µL\u003cbr\u003e\nRabbit Polyclonal GAPDH antibody. Suitable for ELISA, I-ELISA, ICC\/IF and reacts with Enterobacteria phage M13 samples. Cited in 10 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Enterobacteria phage M13,\u003cbr\u003e\nApplications:ICC\/IF, I-ELISA, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nConcentration: 5.5 mg\/ml- 7.5 mg\/ml\u003cbr\u003e\nCan be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in \"phage ELISA\" offering sensitive and specific activity for detection of recombinant phages.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purity-IgG fraction, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe M13 bacteriophage coat proteins also known as the phage display proteins play an important role in the structure and functionality of the M13 phage. Commonly referred to as filamentous bacteriophage proteins these coat proteins include pVIII the major coat protein along with pIII pVI pVII and pIX which are minor coat proteins. The mass of pVIII is approximately 5kDa. These coat proteins are expressed on the surface of the M13 bacteriophage primarily after infection of host bacteria like Escherichia coli facilitating the assembly and stability of the phage particle.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nM13 phage coat proteins are essential in the assembly and secretion process of the bacteriophage. These proteins form a cylindrical structure encasing the single-stranded DNA core critical for the infection cycle of the virus. During M13 phage display technique scientists exploit these coat proteins to present foreign proteins or peptides on the surface. This capability allows these phage particles to function as tools for molecular library screening and antibody engineering. Although not part of a traditional multiprotein complex they work coordinately to maintain phage integrity and facilitate propagation.\u003cbr\u003e\nPathways\u003cbr\u003e\nM13 phage coat proteins are integral to the phage assembly and secretion pathways. These pathways occur in the bacterial host and ensure the correct encapsulation of the single-stranded DNA genome. Related proteins in these processes include bacterial proteins that participate in host cell machinery like the Tol and Pal system which interact with coat proteins like pIII during the phage infection cycle. These interactions are fundamental for the phage's continued life cycle and its use in biotechnological applications like phage ELISA.\u003cbr\u003e\nM13 phage coat proteins have potential implications in cancer and infectious disease research. The M13 bacteriophage due to these coat proteins' adaptability has been used to create phage display libraries aiding in the discovery of biomarkers and target molecules. These coat proteins have been linked with tumor-targeting peptides improving cancer diagnostic and therapeutic strategies. Furthermore researchers have explored their connection with other proteins in immunology for vaccine development against pathogens. This connection highlights their significance in experimental therapeutics.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855809138857,"sku":"ab6188","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab6188","provider":"Iright","version":"1.0","type":"link"}