{"product_id":"abcam-ab62751","title":"Abcam, ab62751, Anti-YAP1 (phospho Y357) antibody","description":"\u003cp\u003eSize: 50µL\u003cbr\u003e\nAnti-YAP1 (phospho Y357) antibody (ab62751) is a rabbit polyclonal antibody detecting YAP1 in  Western Blot, ICC\/IF . Suitable for  Human . - Over 50 publications - Trusted since 2008\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, ICC\/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human YAP1 phospho Y357 aa 350-400 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P46937\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-YAP1 (phospho Y357) antibody (ab62751) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of YAP1?\u003cbr\u003e\nAnti-YAP1 (phospho Y357) (ab62751) specifically detects a band for YAP1 (UniProt: P46937) at a molecular weight of 65kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-YAP1 (phospho Y357) (ab62751) was first used in a scientific publication in 2008 and has been cited over 50 times in peer-reviewed journals.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab62751 was affinity-purified using the immunizing peptide immobilized on agarose., Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nYAP1 also known as Yes-associated protein 1 is a transcriptional coactivator involved in regulating gene expression. This protein has a mass of approximately 65 kDa and is expressed in various tissues including the liver and heart. YAP1 plays a significant role in the Hippo signaling pathway where it acts by binding to transcriptional enhancer factors such as TEADs to activate gene transcription.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nYAP1 influences cell proliferation and apoptosis by interacting with other proteins to form transcriptional complexes. It participates in promoting organ size and tissue regeneration. YAP1's activity is regulated through phosphorylation primarily at serine 127 which determines its subcellular localization and transcriptional activity. The phosphorylated form often referred to as phospho-YAP remains in the cytoplasm while dephosphorylated YAP1 translocates to the nucleus where it contributes to transcriptional activation.\u003cbr\u003e\nPathways\u003cbr\u003e\nYAP1 is importantly involved in the Hippo signaling pathway and Wnt\/Β-catenin signaling pathway. In the Hippo pathway kinases LATS1\/2 phosphorylate YAP1 preventing its nuclear localization and promoting cell apoptosis when required. In contrast in the Wnt\/Β-catenin pathway YAP1 interacts with Β-catenin to regulate gene expression related to proliferation. Proteins such as TAZ which shares structural and functional similarities with YAP1 often collaborate in these pathways influencing cellular growth and differentiation.\u003cbr\u003e\nYAP1's dysregulation links to cancer development notably in liver and breast cancer. Overexpression or nuclear accumulation of YAP1 leads to uncontrollable cell growth and tumorigenesis. Its interaction with the TEAD transcription factors highlights its significance in oncogenesis. Furthermore YAP1 also connects with pathways involving other proteins like TGF-Β contributing to fibrosis-related disorders such as pulmonary fibrosis where unexpected remodeling and excessive tissue growth occur.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848610730153,"sku":"ab62751","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab62751","provider":"Iright","version":"1.0","type":"link"}