{"product_id":"abcam-ab64218","title":"Abcam, ab64218, Hydrogen Peroxide Blocking Reagent","description":"\u003cp\u003eSize: 60mL \/ 125mL\u003cbr\u003e\nHydrogen peroxide blocking reagent (ab64218) is a ready-to-use reagent used to block endogenous peroxidase activity in IHC applications, enhancing signal detection in HRP \/ peroxidase-based systems. - Proven performance: cited in over 40 publications - Available in different sizes to fit your experimental needs - Compatible with our Lab essentials imaging workflow offering\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:Preservative: 0.1% Sodium azideConstituents: 0.3% Hydrogen peroxide\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHydrogen Peroxide Blocking Reagent ab64218 for use in IHC with detection based on HRP \/ peroxidase.\u003cbr\u003e\nNote: The concentration of hydrogen peroxide in this product is 0.3%.\u003cbr\u003e\nIHC protocol suitable for use with Hydrogen Peroxide Blocking Reagent:\u003cbr\u003e\nFor frozen sections, skip steps 1 and 2.\u003cbr\u003e\n1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.\u003cbr\u003e\n2. Use appropriate\u003cbr\u003e\nantigen retrieval buffer or enzyme\u003cbr\u003e\n(primary antibody dependent) to treat sections. Wash 3 times in buffer.\u003cbr\u003e\nAdd enough hydrogen peroxide blocking solution ab64218 to cover the sections. Incubate for 10 minutes.\u003cbr\u003e\nWash 2 times in buffer. If necessary, use\u003cbr\u003e\navidin biotin blocking\u003cbr\u003e\n4. Apply\u003cbr\u003e\nprotein block\u003cbr\u003e\nnormal serum\u003cbr\u003e\nfrom same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.\u003cbr\u003e\n5. Apply primary antibody in\u003cbr\u003e\nantibody diluent\u003cbr\u003e\nand incubate.\u003cbr\u003e\n6. Wash 4 times in buffer. Incubate slide with\u003cbr\u003e\nbiotinylated secondary antibody\u003cbr\u003e\nHRP polymer secondary antibody\u003cbr\u003e\nand skip step 7). Wash 4 times in buffer.\u003cbr\u003e\n7. Apply\u003cbr\u003e\nstreptavidin-HRP\u003cbr\u003e\nand incubate for 10 minutes at room temperature.\u003cbr\u003e\n8. Rinse 4 times in buffer. Place slide in\u003cbr\u003e\nDAB substrate\u003cbr\u003e\nAEC substrate\u003cbr\u003e\nand incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.\u003cbr\u003e\n9. Add enough drops of\u003cbr\u003e\nhematoxylin\u003cbr\u003e\nto cover the section. Incubate for 1 minute.\u003cbr\u003e\n10. Rinse 7-8 times in tap water. Add\u003cbr\u003e\nmounting medium\u003cbr\u003e\nto cover the section.\u003cbr\u003e\nFind complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847571198121,"sku":"ab64218","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab64218","provider":"Iright","version":"1.0","type":"link"}