{"product_id":"abcam-ab64226","title":"Abcam, ab64226, Protein Block","description":"\u003cp\u003eSize: 125mL\u003cbr\u003e\nProtein block (ab64226) is a ready-to-use, serum-free blocking buffer designed to reduce non-specific background staining in IHC, ELISA, and western blot (WB) applications. - Compatible with Mouse specific HRP\/DAB Detection IHC Kit (ab64259) - Proven performance: cited in over 130 publications - Compatible with our Lab essentials imaging and staining workflow offering\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:WB, ELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 7.2 - 7.6Preservative: 0.1% Sodium azideConstituents: PBS, 0.5% BSA, 0.5% Casein\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProtein Block has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining while simultaneously reducing the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. Protein Block has been shown to be effective for immunohistochemical, ELISA, and blot and requires no mixing or diluting.\u003cbr\u003e\nProtein blocking buffer ab64226 for serum-free blocking of non-specific antibody binding in IHC, ELISA and western blot. Ready to use. No mixing or dilution required.\u003cbr\u003e\nFor use in ELISA, add Protein Block to well and incubate for 2-10 minutes before addition of sample. Wash and continue procedure. For use in western blotting, incubate for one hour at room temperature or overnight at 4°C.\u003cbr\u003e\nIHC protocol suitable for use with Protein Block ab64226:\u003cbr\u003e\nFor frozen sections, skip steps 1 and 2. For fluorescent IHC, skip step 3, incubate with fluorescent dye conjugated secondary at step 6, skip rest of steps, and mount with\u003cbr\u003e\nanti-fade mounting medium\u003cbr\u003e\n1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.\u003cbr\u003e\n2. Use appropriate\u003cbr\u003e\nantigen retrieval buffer or enzyme\u003cbr\u003e\n(primary antibody dependent) to treat sections. Wash 3 times in buffer.\u003cbr\u003e\n3. Add enough\u003cbr\u003e\nhydrogen peroxide blocking solution\u003cbr\u003e\nto cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use\u003cbr\u003e\navidin biotin blocking\u003cbr\u003e\nApply Protein Block ab64226\u003cbr\u003e\n(or normal serum from same species as secondary antibody)\u003cbr\u003e\nand incubate for at least 30 minutes at room temperature\u003cbr\u003e\nto block nonspecific background staining. Wash once in buffer.\u003cbr\u003e\n5. Apply primary antibody in\u003cbr\u003e\nantibody diluent\u003cbr\u003e\nand incubate. Wash 4 times in buffer.\u003cbr\u003e\n6. Incubate with\u003cbr\u003e\nbiotinylated secondary antibody\u003cbr\u003e\nHRP polymer secondary antibody\u003cbr\u003e\nand skip step 7). Wash 4 times in buffer.\u003cbr\u003e\n7. Apply\u003cbr\u003e\nstreptavidin-HRP\u003cbr\u003e\nand incubate for 10 minutes at room temperature.\u003cbr\u003e\n8. Rinse 4 times in buffer. Place slide in\u003cbr\u003e\nDAB substrate\u003cbr\u003e\nAEC substrate\u003cbr\u003e\nand incubate until desired color is achieved. Rinse 4 times in buffer.\u003cbr\u003e\n9. Add enough drops of\u003cbr\u003e\nhematoxylin\u003cbr\u003e\nto cover the section. Incubate for 1 minute.\u003cbr\u003e\n10. Rinse 7-8 times in tap water. Add\u003cbr\u003e\nmounting medium\u003cbr\u003e\nto cover the section.\u003cbr\u003e\nFind complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847571919017,"sku":"ab64226","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab64226","provider":"Iright","version":"1.0","type":"link"}