{"product_id":"abcam-ab64257","title":"Abcam, ab64257, Biotinylated Goat anti-Mouse \u0026 Rabbit IgG (H+L) (Ready To Use)","description":"\u003cp\u003eSize: 125mL\u003cbr\u003e\nSuitable for IHC-P. Cited in 10 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Goat,\u003cbr\u003e\nTarget species:Rabbit,\u003cbr\u003e\nTarget isotype:IgG,\u003cbr\u003e\nTarget specificity:Heavy \u0026amp; Light chains,\u003cbr\u003e\nPre-adsorbed:No,\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nClonality:Polyclonal\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nStaining protocol\u003cbr\u003e\nDeparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.\u003cbr\u003e\nAdd enough drops of an appropriate hydrogen peroxide block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.\u003cbr\u003e\nPerform appropriate pretreatment if required. Wash 3 times in buffer.\u003cbr\u003e\nApply an appropriate protein block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.\u003cbr\u003e\nApply primary antibody and incubate according to manufacturer's protocol.\u003cbr\u003e\nWash 4 times in buffer. Apply Biotinylated Goat anti Mouse \u0026amp; Rabbit IgG(H+L) solution (ab64257) and incubate for 10 minutes at room temperature. Wash 4 times in buffer.\u003cbr\u003e\nApply enzyme labeled streptavidin and incubate according to manufacturer's protocol.\u003cbr\u003e\nRinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.\u003cbr\u003e\nAdd enough drops of Hematoxylin to cover the section. Incubate for 1 minute.\u003cbr\u003e\nRinse 7-8 times in tap water. Add Mounting Medium to cover the section.\u003cbr\u003e\nThis reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-pH: 7.2 - 7.6Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847538921641,"sku":"ab64257","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab64257","provider":"Iright","version":"1.0","type":"link"}