{"product_id":"abcam-ab64269","title":"Abcam, ab64269, Streptavidin HRP (ready-to-use)","description":"\u003cp\u003eSize: 125mL\u003cbr\u003e\nStreptavidin HRP (ready-to-use) (ab64269) reagent is used to detect biotinylated antibodies in immunohistochemistry (IHC) assays. - Proven performance: cited in over 60 publications - Forms a strong, specific non-covalent complex with biotin - Has less non-specific binding and less background signal compared to avidin - Compatible with our Lab essentials imaging workflow offering\u003cbr\u003e\nKey facts\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:No,\u003cbr\u003e\nAccession:P22629,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Streptomyces avidinii,\u003cbr\u003e\nStorage buffer:pH: 7 - 8Preservative: 0.2% Proclin 300Constituents: PBS, 9% Goat Serum, 1.08% BSA, 0.05% Sorbitan monolaurate, ethoxylated, 0.012% HRP-conjugated streptavidin\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nStreptavidin-HRP for use with biotinylated secondary antibodies during IHC \/ immunohistochemistry. Optimized, ready-to-use format.\u003cbr\u003e\nThis product was previously called Streptavidin-Peroxidase.\u003cbr\u003e\nIHC protocol suitable for use with Streptavidin-HRP ab64269:\u003cbr\u003e\n1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.\u003cbr\u003e\n2. Use appropriate\u003cbr\u003e\nantigen retrieval buffer or enzyme\u003cbr\u003e\n(primary antibody dependent) to treat sections. Wash 3 times in buffer.\u003cbr\u003e\n3. Add enough\u003cbr\u003e\nhydrogen peroxide blocking solution\u003cbr\u003e\nto cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use\u003cbr\u003e\navidin biotin blocking\u003cbr\u003e\n4. Apply\u003cbr\u003e\nprotein block\u003cbr\u003e\n(or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.\u003cbr\u003e\n5. Apply primary antibody in\u003cbr\u003e\nantibody diluent\u003cbr\u003e\nand incubate.\u003cbr\u003e\n6. Wash 4 times in buffer. Incubate slide with\u003cbr\u003e\nbiotinylated secondary antibody\u003cbr\u003e\nHRP polymer secondary antibody\u003cbr\u003e\nand skip step 7). Wash 4 times in buffer.\u003cbr\u003e\nApply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.\u003cbr\u003e\n8. Rinse 4 times in buffer. Place slide in\u003cbr\u003e\nDAB substrate\u003cbr\u003e\nAEC substrate\u003cbr\u003e\nand incubate until desired color is achieved. Rinse 4 times in buffer.\u003cbr\u003e\n9. Add enough drops of\u003cbr\u003e\nhematoxylin\u003cbr\u003e\nto cover the section. Incubate for 1 minute.\u003cbr\u003e\n10. Rinse 7-8 times in tap water. Add\u003cbr\u003e\nmounting medium\u003cbr\u003e\nto cover the section.\u003cbr\u003e\nFind complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843797438633,"sku":"ab64269","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab64269","provider":"Iright","version":"1.0","type":"link"}