{"product_id":"abcam-ab65330","title":"Abcam, ab65330, L-Lactate Assay Kit (Colorimetric\/Fluorometric)","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nL-Lactate Assay Kit (Colorimetric\/Fluorometric) ab65330 is a quantitative, addition-only assay with just one 30 min incubation step at room temperature. Readout on any colorimetric (570 nm) or fluorometric (Ex\/Em 360nm\/440nm) plate reader. - Cited in over 250 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Tissue Lysate, Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 0.001 mM,\u003cbr\u003e\nRange:0.001 - 10 mM,\u003cbr\u003e\nAssay time:40m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nL-Lactate Assay Kit (Colorimetric\/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc. There is no need for pretreatment or purification of samples.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nIn the lactate assay protocol, lactate specifically reacts with an enzyme mix to generate a product, which interacts with a lactate probe to produce color (570 nm) and fluorescence (at Ex\/Em = 535\/587 nm).\u003cbr\u003e\nLactate assay protocol summary\u003cbr\u003e\n- Add samples (deproteinized) and standards to wells.\u003cbr\u003e\n- Add reaction mix and incubate for 30 min at room temp.\u003cbr\u003e\n- Analyze with microplate reader.\u003cbr\u003e\nRelated Lactate assay products\u003cbr\u003e\nAlternative L-Lactate assay kits offer different readout modes\/wavelengths and sensitivity\/range:\u003cbr\u003e\n- This kit L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex\/Em 535\/587 nm, range 0.001 mM - 10 mM.\u003cbr\u003e\n- Our most popular L-Lactate assay kit\u003cbr\u003e\nab65331\u003cbr\u003e\n: colorimetric 450nm, range 0.02 mM - 10 mM.\u003cbr\u003e\n- L-Lactate assay kit\u003cbr\u003e\nab169557\u003cbr\u003e\n: fluorometric Ex\/Em 535\/587 nm, range 0.2 µM - 50 µM.\u003cbr\u003e\nL(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.\u003cbr\u003e\nWe also provide D-Lactate assay kit\u003cbr\u003e\nab83429\u003cbr\u003e\nLactate assay methods\u003cbr\u003e\nThere are two lactate assay methods established in biological research:\u003cbr\u003e\na) The most commonly used method, where lactate dehydrogenase processes lactate in the presence of NAD+ to produce pyruvate and NADH. NADH then reduces a probe to form a colorimetric or fluorometric readout. This method, with some improvements, is used in\u003cbr\u003e\nab65331\u003cbr\u003e\nand in\u003cbr\u003e\nab169557\u003cbr\u003e\nb) The alternative method, where lactate oxidase processes lactate to produce pyruvate and hydrogen peroxide. This is followed by oxidation of a substrate by a peroxidase using the hydrogen peroxide to produce a colorimetric or fluorometric readout. This method is used in ab65330.\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K607 Lactate Colorimetric\/Fluorometric Assay Kit. K607-100 is the same size as the 100 test size of ab65330.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nL-Lactate also known as lactate is a byproduct of anaerobic glycolysis where it plays an important role in energy metabolism. L-Lactate is a small molecule with a molecular mass of approximately 90.08 g\/mol. It forms in various tissues like muscle cells during intense exercise when oxygen levels are low. This process leads to a conversion of pyruvate to lactate by the action of the enzyme lactate dehydrogenase (LDH) which is present in many tissues with higher expressions in muscles and heart.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nL-Lactate acts as a signaling molecule which affects cellular functions and contributes to metabolic regulation. It is not part of a complex but serves as an important intermediate in metabolic pathways. L-Lactate provides energy to cells by converting back to pyruvate in the presence of oxygen which then enters the citric acid cycle. This conversion and its utilization as energy play important roles in balancing cellular energy demands especially under hypoxic conditions.\u003cbr\u003e\nPathways\u003cbr\u003e\nL-Lactate links to critical processes like glycolysis and the Cori cycle. During glycolysis pyruvate may convert to L-Lactate under anaerobic conditions to regenerate NAD+ necessary for glycolysis to continue. In the Cori cycle lactate produced by anaerobic glycolysis in muscles is released into the bloodstream and transported to the liver. There it converts back to glucose supporting gluconeogenesis. These processes highlight the close involvement of L-Lactate with proteins such as lactate dehydrogenase and pyruvate kinase.\u003cbr\u003e\nL-Lactate is associated with conditions like lactic acidosis and cancer. Lactic acidosis characterized by high lactate levels can occur due to oxygen deprivation or mitochondrial dysfunction. Meanwhile cancer cells often show enhanced glycolysis and lactate production known as the Warburg effect facilitating their growth. Proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDH) are key players in these conditions influencing lactate metabolism and potentially serving as therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843537817769,"sku":"ab65330","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab65330","provider":"Iright","version":"1.0","type":"link"}