{"product_id":"abcam-ab65339","title":"Abcam, ab65339, Creatine Assay Kit (Colorimetric\/Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nCreatine Assay Kit ab65339 is a quantitative, mix-and-read assay with one 60-min incubation step. In the assay, creatine is acted on by creatinase, followed by the action of sarcosine oxidase and HRP. Readout on any colorimetric (570 nm) or fluorometric (Ex\/Em 538\/587 nm) plate reader. - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026gt; 0.001 mM,\u003cbr\u003e\nRange:0.001 - 10 mM,\u003cbr\u003e\nAssay time:1h,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCreatine Assay Kit (Colorimetric\/Fluorometric) (ab65339) provides an accurate, convenient measure of creatine in a variety of biological samples.\u003cbr\u003e\nCreatine assay principle\u003cbr\u003e\nIn the creatine assay, creatine is enzymatically converted to sarcosine by creatinase. Sarcosine is then oxidized by sarcosine oxidase producing hydrogen peroxide. Hydrogen peroxide is then processed by a peroxidase causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex\/Em = 535\/587 nm). Detection is by either colorimetric or fluorometric plate reader.\u003cbr\u003e\nThis is the same method used in the creatinine assay kit\u003cbr\u003e\nab65340\u003cbr\u003e\n, except that this kit ab65339 does not include the initial creatininase enzyme step.\u003cbr\u003e\nCreatine assay protocol summary\u003cbr\u003e\n- add reaction mix to sample and standard wells\u003cbr\u003e\n- incubate for 60 min\u003cbr\u003e\n- analyze with a microplate reader\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nRelated assay kits for the study of creatine and creatinine biology include:\u003cbr\u003e\n- Creatinine assay kit\u003cbr\u003e\nab65340\u003cbr\u003e\n- Creatine kinase activity assay kit\u003cbr\u003e\nab155901\u003cbr\u003e\nSee the product datasheet for\u003cbr\u003e\nab65340\u003cbr\u003e\nfor a description of our assays for measuring the albumin-creatinine ratio and urea creatinine ratio.\u003cbr\u003e\nOther notes\u003cbr\u003e\nThis product was previously called K635 Biovision Creatine Colorimetric\/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nCreatine is an endogenous compound whose function is to maintain a high ATP\/ADP ratio, by way of its phosphorylated form and creatine kinase. Creatine supplementation has been used in the treatment of muscular, neurological and neurodegenerative diseases, as well as a sport performance enhancer. Detection of creatine level has wide applications in research and development.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843619082409,"sku":"ab65339","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab65339","provider":"Iright","version":"1.0","type":"link"}