{"product_id":"abcam-ab65621","title":"Abcam, ab65621, Protease Inhibitor Cocktail","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nProtease inhibitor cocktail is a water-soluble mixture used to inhibit a wide range of proteases during protein extraction, preventing protein degradation.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nAbcam's Protease Inhibitor Cocktail contains a mixture of water-soluble protease inhibitors with broad specificity for various proteases, and has been optimized to generate the maximal protection to mammalian tissue and cell extracts.\u003cbr\u003e\nSupplied as a lyophilized powder.\u003cbr\u003e\nOne vial can make 125 ml of cocktail solution.\u003cbr\u003e\nPreparation:\u003cbr\u003e\nAdd 250 μl DMSO to the vial, pipette several times to dissolve all powder (This makes 500X concentrated inhibitor cocktail).\u003cbr\u003e\nVisit our\u003cbr\u003e\nFAQs page\u003cbr\u003e\nfor tips and troubleshooting.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K271 Protease Inhibitor Cocktail. K271-500 is the same size as the 1 kit size of ab65621.\u003cbr\u003e\nCrude cell extracts contain many endogeneous enzymes, such as proteases and phosphotases, which can degrade valuable proteins in the extract. The individual components of the protease inhibitor cocktail have specific inhibitory properties. A description of each inhibitor is given below:\u003cbr\u003e\nAprotinin:\u003cbr\u003e\nInhibits serine proteases, such as trypsin, chymotrypsin, plasmin, trypsinogen, urokinase, and kallikrein.\u003cbr\u003e\nLeupeptin:\u003cbr\u003e\nInhibits both serine and cysteine proteases, such as calpain, trypsin, papain, and cathepsin B.\u003cbr\u003e\nPepstatin A:\u003cbr\u003e\nInhibits acid proteases, such as pepsin, rennin, cathepsin D, chymosin, and protease B.\u003cbr\u003e\nInhibits serine proteases, such as trypsin and chymotrypsin. Also inhibits cysteine proteases and mammalian acetylcholinesterase.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847586271401,"sku":"ab65621","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab65621","provider":"Iright","version":"1.0","type":"link"}