{"product_id":"abcam-ab66090","title":"Abcam, ab66090, Apoptosis DNA Ladder Assay Kit","description":"\u003cp\u003eSize: 50Test\u003cbr\u003e\nApoptosis DNA Ladder Assay Kit ab66090 provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Direct,\u003cbr\u003e\nAssay time:1h 30m\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nApoptosis DNA Ladder Assay Kit (ab66090) provides an easy and sensitive solution for detecting DNA fragmentation in apoptotic cells. It can isolate small fragmented DNA from cells in only 90 minutes. No DNA extraction or additional columns required.\u003cbr\u003e\nApoptosis DNA ladder assay protocol summary:\u003cbr\u003e\n- pellet cells by spinning\u003cbr\u003e\n- wash cells with PBS and spinning\u003cbr\u003e\n- lyse cells with TE lysis buffer and pipetting\u003cbr\u003e\n- add enzyme A solution and incubate for 10 min\u003cbr\u003e\n- add enzyme B solution and incubate for 30 min\u003cbr\u003e\n- add ammonium acetate solution, isopropanol and freeze for 10 min\u003cbr\u003e\n- spin to preciptate DNA\u003cbr\u003e\n- wash pellet with 70% ethanol, air-dry and dissolve in DNA suspension buffer\u003cbr\u003e\n- run on agarose gel and visualize with DNA staining dye\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called Apoptotic DNA Ladder Detection Kit.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843548926121,"sku":"ab66090","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab66090","provider":"Iright","version":"1.0","type":"link"}