{"product_id":"abcam-ab66108","title":"Abcam, ab66108, TUNEL Assay Kit - FITC","description":"\u003cp\u003eSize: 50Test\u003cbr\u003e\nTUNEL Assay Kit - FITC ab66108 allows detecting DNA fragmentation by fluorescence microscopy or flow cytometry. - Simple and fast protocol for cultured cells and tissue samples - Cited in \u0026gt;80 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Direct,\u003cbr\u003e\nAssay time:2h 30m,\u003cbr\u003e\nAssay Platform:Flow cytometer, Fluorescence microscope\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nTUNEL assay - FITC ab66108 is used to detect DNA fragmentation in apoptosis.\u003cbr\u003e\nThe TUNEL assay - FITC used to detect DNA fragmentation, such as in apoptosis. It uses terminal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of deoxynucleotides at the free 3'-hydroxyl ends of fragmented DNA. The deoxynucleotides are then labeled in a variety of ways for detection of the degree of DNA fragmentation.\u003cbr\u003e\nThe TUNEL assay protocol in this kit uses the deoxynucleotide fluorescein-12-dUTP. DNA which has been labeled with fluorescein can then be analyzed by flow cytometry or fluoresence microscopy with Ex\/Em 488\/520 nm. Propidium iodide is also included in this kit as a counterstain with Ex\/Em 488\/623 nm.\u003cbr\u003e\nTUNEL assay protocol summary:\u003cbr\u003e\n- fix cells with formaldehyde for 15 min on ice\u003cbr\u003e\n- wash with PBS\u003cbr\u003e\n- add ice-cold 70% ethanol and incubate for 30 min\u003cbr\u003e\n- pellet cells and resuspend in wash buffer and wash again\u003cbr\u003e\n- pellet cells and resuspend in staining solution and incubate for 60 min at 37°C\u003cbr\u003e\n- add rinse buffer, pellet cells and discard supernatant, and rinse again\u003cbr\u003e\n- resuspend cells in propidium iodide\/RNAse A solution and incubate for 30 min at room temp\u003cbr\u003e\n- analyze with flow cytometer or fluoresence microscope\u003cbr\u003e\nTo use HRP detection, we recommend TUNEL Assay Kit - HRP-DAB\u003cbr\u003e\nab206386\u003cbr\u003e\n. To use BrdU-Red (Ex\/Em = 488\/576nm) as a label, we recommend\u003cbr\u003e\nab66110\u003cbr\u003e\nHow other researchers are using\u003cbr\u003e\nTUNEL Assay Kit - FITC has been used in variety of sample type including:\u003cbr\u003e\n- Human lung cancer cell lines A549 and Human colon cancer cell lines HCT-116 1\u003cbr\u003e\n- Mouse lung tissue\u003cbr\u003e\n- Mouse Mesenchymal stem cell (MSC) 3\u003cbr\u003e\nReferences:\u003cbr\u003e\n1 - Olszewski M et al. 2024; 2 - Zhang P et at 2024; 3 - Zhang T et al. 2023\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843596898473,"sku":"ab66108","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab66108","provider":"Iright","version":"1.0","type":"link"}