{"product_id":"abcam-ab70544","title":"Abcam, ab70544, Anti-BubR1 antibody","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nRabbit Polyclonal BubR1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BUB1B.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human BUB1B. The exact immunogen used to generate this antibody is proprietary information.O60566\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nBubR1 also known as BUB1B is an important protein in the mitotic checkpoint complex with an approximate mass of 120 kDa. BubR1 ensures proper chromosome alignment and segregation during cell division by inhibiting the anaphase-promoting complex\/cyclosome (APC\/C) until all chromosomes achieve proper attachment to the spindle. This protein localizes mainly to the kinetochores of chromosomes and is commonly expressed in dividing cells throughout the body.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe spindle assembly checkpoint mechanism involves a complex in which BubR1 plays a central role. BubR1 along with other checkpoint proteins such as Bub1 and Mad2 forms a surveillance complex ensuring the correct attachment of microtubules to kinetochores. This action prevents premature progression to anaphase reducing the risk of aneuploidy and maintaining genomic stability.\u003cbr\u003e\nPathways\u003cbr\u003e\nMitotic checkpoint control critically involves BubR1 linking closely with several proteins including Mad2 and Cdc20. BubR1 integrates into the spindle checkpoint pathway a central player in regulating mitosis. Within this pathway BubR1 inhibits APC\/CCdc20 activity preventing premature anaphase onset until all chromosomes are correctly oriented to divide. Interactions between these proteins ensure accurate mitotic progression and cellular replication fidelity.\u003cbr\u003e\nBubR1 function directly impacts cancer development and premature aging syndromes. Defective BubR1 activity can lead to chromosomal instability a characteristic of many cancers including colorectal cancer. Moreover reduced BubR1 expression links with premature aging syndromes such as mosaic variegated aneuploidy through its interaction with proteins such as p53 highlighting its role in genome integrity maintenance and cellular aging processes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46850087551145,"sku":"ab70544","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab70544","provider":"Iright","version":"1.0","type":"link"}