{"product_id":"abcam-ab7468","title":"Abcam, ab7468, NBT \/ BCIP Solution - Ready to Use Alkaline Phosphatase chromogen","description":"\u003cp\u003eSize: 100mL\u003cbr\u003e\nNBT \/ BCIP Solution (ab7468 ) is an easy-to-use liquid substrate for alkaline phosphatase (AP) detection in immunoblotting assays e.g. Western blot. - Ready-to-use solution.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nNBT \/ BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.\u003cbr\u003e\nBCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.\u003cbr\u003e\nThis product used to be called \"Alkaline phosphatase detection kit (BCIP\/NBT)\".\u003cbr\u003e\nNBT \/ BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.\u003cbr\u003e\nTo use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847602065577,"sku":"ab7468","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab7468","provider":"Iright","version":"1.0","type":"link"}