{"product_id":"abcam-ab78","title":"Abcam, ab78, Anti-ATM antibody [2C1 (1A1)] - BSA and Azide free","description":"\u003cp\u003eSize: 100µL\u003cbr\u003e\nAnti-ATM antibody [2C1 (1A1)] - BSA and Azide free (ab78) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting ATM in  Western Blot, Flow Cytometry, IP, IHC-P . Suitable for  Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Over 110 publications - Trusted since 2002\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:2C1 (1A1),\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:IHC-P, WB, IP, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Recombinant Fragment Protein within Human ATM aa 2550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q13315,\u003cbr\u003e\nSpecificity:The ATM antibody, clone 2C1, recognizes full-length ATM.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-ATM antibody [2C1 (1A1)] - BSA and Azide free (ab78) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.\u003cbr\u003e\nWhat is the molecular weight of ATM?\u003cbr\u003e\nAnti-ATM [2C1 (1A1)] - BSA and Azide free (ab78) specifically detects a band for ATM (UniProt: Q13315) at a molecular weight of 350kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-ATM [2C1 (1A1)] - BSA and Azide free (ab78) was first used in a scientific publication in 2002 and has been cited over 110 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-ATM [2C1 (1A1)] - BSA and Azide free (ab78) has over 10 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification notes-Purified from TCS by Protein G chromatography to at least 95% homogeneity as determined by SDS-PAGE., Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.\u003cbr\u003e\nPathways\u003cbr\u003e\nATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.\u003cbr\u003e\nATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46848575045801,"sku":"ab78","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab78","provider":"Iright","version":"1.0","type":"link"}