{"product_id":"abcam-ab80289","title":"Abcam, ab80289, Recombinant human KMT1A \/ SUV39H1 protein","description":"\u003cp\u003eSize: 50µg\u003cbr\u003e\nRecombinant human KMT1A \/ SUV39H1 protein is a Human Fragment protein, in the 82 to 412 aa range, expressed in Escherichia coli, with \u0026gt;50%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;50% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific Activity: 3.5 pmol\/min\/μg.Assay conditions: 50 μl reaction mix (50 mM TRIS pH 8.8, 5mM MgCl2, 4mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and 10-100 ng SUV39H1) add to the wells coated with the substrate on a GBioscience Neutravidin black plate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody andincubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.,\u003cbr\u003e\nAccession:O43463,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.395% Tris HCl, 0.05% Sorbitan monolaurate, ethoxylated, 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nKMT1A also known as SUV39H1 is a well-studied histone methyltransferase responsible for trimethylating histone H3 at lysine 9 (H3K9me3) an important marker for heterochromatin formation. This enzyme plays a critical role in chromatin organization and gene regulation. KMT1A has a molecular weight of approximately 48 kDa. Researchers have observed its expression across a range of tissues including the brain heart and muscle which suggests its importance in various physiological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nKMT1A functions as part of a Suv39H1\/HP1 complex that maintains the integrity of pericentric heterochromatin essential for chromosome stability during cell division. Its influence extends over the transcriptional silencing of specific genes by modifying chromatin structure to a closed state. KMT1A also collaborates with other proteins like HP1 to silence repetitive elements and prevent genomic instability which is often important for safeguarding genomic fidelity in cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nResearchers identify KMT1A as a significant player in the regulation of epigenetic pathways particularly the histone methylation process. It interacts closely with other histone methyltransferases and demethylases contributing to a dynamic epigenetic landscape that controls gene expression. Additionally KMT1A is involved in the DNA damage response pathway in collaboration with proteins like ATM which coordinates repair mechanisms to maintain genetic stability and respond to cellular stress.\u003cbr\u003e\nResearchers have linked KMT1A with cancer and neurodegenerative diseases such as Huntington’s disease. Abnormal expression or function of KMT1A can lead to disrupted heterochromatin structure and uncontrolled gene expression which contributes to tumorigenesis. Furthermore in the context of neurodegenerative conditions interactions with proteins such as HTT can exacerbate neuronal damage and cell death. Studying these associations provides insights into potential therapeutic targets for managing such diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843837513897,"sku":"ab80289","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab80289","provider":"Iright","version":"1.0","type":"link"}