{"product_id":"abcam-ab81461","title":"Abcam, ab81461, Anti-GLO1 antibody [6F10]","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nRat Monoclonal GLO1 antibody. Suitable for WB, ICC\/IF, Flow Cyt (Intra) and reacts with Mouse, Human, African green monkey samples. Cited in 13 publications.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rat,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:6F10,\u003cbr\u003e\nIsotype:IgG2b,\u003cbr\u003e\nLight chain type:kappa,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Human, African green monkey,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab81461 also reacts with Simian.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification notes-The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. It is filter sterilized., Storage buffer-pH: 6 - 8.5Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe GLO1 protein also known as glyoxalase I plays an important role in cellular metabolism by detoxifying methylglyoxal a byproduct of glycolysis. It catalyzes the conversion of methylglyoxal into S-D-lactoylglutathione using glutathione as a cofactor. This enzyme has a mass of approximately 29 kDa and is expressed in various tissues throughout the body with higher levels found in the liver and kidney. Glyoxalase I (GLO1) expression is increased in response to stress conditions indicating its role in cellular defense mechanisms.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlyoxalase I is important for maintaining cellular homeostasis by mitigating harmful compounds. It forms part of the glyoxalase system which includes glyoxalase II functioning downstream of GLO1. This system prevents the accumulation of advanced glycation end-products (AGEs) which are implicated in cellular aging and dysfunction. By reducing the levels of methylglyoxal GLO1 supports normal physiological processes and protects cellular integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlyoxalase I is an important component of the glyoxalase pathway. This pathway integrates into the detoxification network working alongside other important enzymes such as superoxide dismutase and catalase. These relationships highlight its function in cellular oxidative stress responses. GLO1's activity impacts pathways related to glycolysis and overall energy metabolism due to its role in reducing metabolic byproducts.\u003cbr\u003e\nGLO1 has significant implications in diabetic complications and cancer. In diabetes increased methylglyoxal can worsen tissue damage making GLO1's role protective against advanced glycation end-product formation. In cancer GLO1 is overexpressed contributing to cellular proliferation and survival. Its interaction with proteins such as Hsp70 in cancer illustrates the adaptive mechanisms that tumor cells use for progression and chemoresistance.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855474086057,"sku":"ab81461","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab81461","provider":"Iright","version":"1.0","type":"link"}