{"product_id":"abcam-ab818","title":"Abcam, ab818, Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) is a mouse monoclonal antibody detecting TATA binding protein TBP in  Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC\/IF, ELISA, EM, ChIP . Suitable for  Human, Mouse, Rat . - Over 260 publications - Trusted since 2002\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:1TBP18,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Human, Mouse, Rat,\u003cbr\u003e\nApplications:Flow Cyt, ChIP, IHC-P, ELISA, ICC\/IF, IHC-Fr, WB, IP, ChIP\/Chip, ICC, EMSASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:Synthetic Peptide within Human TBP. The exact immunogen used to generate this antibody is proprietary information.P20226,\u003cbr\u003e\nEpitope:Within amino acid residues 1-20 of human, mouse and rat TBP.The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry\/immunofluorescence (ICC\/IF), ELISA, Electron microscopy (EM), ChIP in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of TATA binding protein TBP?\u003cbr\u003e\nAnti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) specifically detects a band for TATA binding protein TBP (UniProt: P20226) at a molecular weight of 38kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) was first used in a scientific publication in 2002 and has been cited over 260 times in peer-reviewed journals.\u003cbr\u003e\nReviewed by scientists\u003cbr\u003e\nAnti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) has over 35 independent reviews from customers.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.\u003cbr\u003e\nPathways\u003cbr\u003e\nTBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.\u003cbr\u003e\nTBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844244557993,"sku":"ab818","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab818","provider":"Iright","version":"1.0","type":"link"}