{"product_id":"abcam-ab83464","title":"Abcam, ab83464, Catalase Activity Assay Kit (Colorimetric\/Fluorometric)","description":"\u003cp\u003eSize: 100Test \/ 2000Test\u003cbr\u003e\nCatalase Activity Assay Kit (Colorimetric\/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids. Readout on any colorimetric (570 nm) or fluorometric (Ex\/Em 535\/587 nm) plate reader. - Cited in \u0026gt; 120 publications - Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric\/Fluorometric,\u003cbr\u003e\nSample types:Tissue Lysate, Plasma, Serum, Cell Lysate,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nAssay time:40m,\u003cbr\u003e\nAssay Platform:Microplate reader\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCatalase Activity Assay Kit (Colorimetric\/Fluorometric) (ab83464) is a highly sensitive, simple and direct assay for measuring catalase activity in a variety of biological samples such as cell and tissue lysates or biological fluids.\u003cbr\u003e\nIn the catalase activity assay protocol, the catalase present in the sample reacts with hydrogen peroxide (H\u003cbr\u003e\n) to produce water and oxygen. The unconverted H\u003cbr\u003e\nreacts with probe to produce a product that can be measured colorimetrically at OD 570 nm or fluorometrically at Ex\/Em = 535\/587 nm. Therefore, the catalase activity present in the sample is reversely proportional to the signal obtained. The kit can detect as little as 1 μU of catalase activity.\u003cbr\u003e\nCatalase activity assay protocol summary:\u003cbr\u003e\n- add samples and standards to wells\u003cbr\u003e\n- add stop solution into sample control wells and incubate at 25°C for 5 min\u003cbr\u003e\n- add H2O2 solution into wells and incubate for 30 min at 25°C\u003cbr\u003e\n- add stop solution\u003cbr\u003e\n- add developer mix and incubate for 10 min at 25°C\u003cbr\u003e\n- analyze with microplate reader\u003cbr\u003e\nTechnical Note: This assay is not recommended for use in urine samples due to the presense of reducing agents.\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K773 Catalase Activity Colorimetric\/Fluorometric Assay Kit. K773-100 is the same size as the 100 test size of ab83464.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCatalase also known as CAT is an enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen. This enzyme has a molecular weight of approximately 240 kDa and typically forms a tetramer. Catalase mainly resides in peroxisomes functioning as a peroxisome marker. It expresses abundantly in the liver kidneys and erythrocytes where it plays significant roles in cellular protection against oxidative damage. The presence of Catalase makes it an ideal candidate for use in peroxisome staining and peroxisome image analysis in research.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCatalase contributes to antioxidant defense by breaking down hydrogen peroxide preventing cellular damage. In peroxisomes it works alongside other peroxisomal enzymes to maintain cell health and metabolic regulation. Catalase does not form a complex but interacts closely with other enzymes like superoxide dismutase which dismutates superoxide radicals into less harmful substances. Increased Catalase activity levels can be measured using Catalase activity kits and activity assays allowing us to learn about peroxisome function.\u003cbr\u003e\nPathways\u003cbr\u003e\nHydrogen peroxide removal by Catalase is vital in the reactive oxygen species (ROS) metabolic process and plays a part in the cellular response to oxidative stress. Catalase interacts with the glutathione peroxidase pathway safeguarding cells from oxidative stress-related damage. Superoxide dismutase works synergistically with Catalase transforming superoxide anions into hydrogen peroxide before its decomposition by Catalase. These activities highlight the essential role Catalase plays in protecting cells from oxidative stress damage.\u003cbr\u003e\nCatalase relates to conditions like acatalasemia and diabetes. Acatalasemia a condition caused by a deficiency of Catalase increases the risk of developing diabetes and other oxidative stress-related diseases. Mutations in the CAT gene can lead to decreased Catalase activity contributing to the onset of these conditions. Additionally Catalase works with other proteins like glutathione peroxidase in mitigating the effects of oxidative stress with deficiencies potentially exacerbating complications in diabetes management.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843542438057,"sku":"ab83464","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab83464","provider":"Iright","version":"1.0","type":"link"}