{"product_id":"abcam-ab86219","title":"Abcam, ab86219, Recombinant human Cyclophilin A protein (Active)","description":"\u003cp\u003eSize: 100µg \/ 500µg\u003cbr\u003e\nRecombinant human Cyclophilin A protein (Active) is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with \u0026gt;95%, \u0026lt; 1 EU\/µg endotoxin level, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific activity is \u0026gt;650 nmoles\/min\/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.Activity AssayPrepare 180μl assay buffer into a suitable container:Test is 120μl of 200mM Tris-HCl pH8.0, 50μl of 20nM chymotrypsin and 10μl of 100μg\/ml Cyclophilin A protein.Blank is 130μl of 200mM Tris-HCl pH8.0 and 50μl of 20nM chymotrypsin.Equilibrate to 37°C and monitor the A405nm until value is constant using a spectrophotometer.Load 20μl of 5mM Suc-AAPF-pNA into plate. (460mM Lithium chloride, 3mM 2,2,2-Trifluoroethanol in DW. The suc-AAPF-pNA is first dissolved in DMSO and diluted once in 200 mM Tris-HCl (pH8.0). Finally, manufacture the final 5mM with 460mM Lithium chloride, 3mM 2,2,2-Trifluoroethanol).Add 180μl of assay buffer in 5mM Suc-AAPF-pNA.Record the absorbance A405nm for 40 minutes at 37 °C.,\u003cbr\u003e\nAccession:P62937,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.116% Sodium chloride, 0.0077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMW confirme by MALDI-TOF.\u003cbr\u003e\nConcentration determined by Bradford assay.\u003cbr\u003e\nEndotoxin levels determined by LAL method.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843808088233,"sku":"ab86219","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab86219","provider":"Iright","version":"1.0","type":"link"}