{"product_id":"abcam-ab9108","title":"Abcam, ab9108, Anti-6X His tag® antibody","description":"\u003cp\u003eSize: 100µg\u003cbr\u003e\nAnti-6X His tag® antibody (ab9108) is a rabbit polyclonal antibody detecting 6X His tag® in  Western Blot, IP, ICC\/IF, ELISA, ChIP . - Over 160 publications - Trusted since 2002\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Polyclonal,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nApplications:WB, ChIP, ICC\/IF, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nSpecificity:ELISA: Antibody specificity was verified by ELISA against peptide conjugated to BSA (HHHHHH-BSA). A 1:25,000 dilution of the antibody gave an OD=1.0 in a 15 minute reaction using HRP-conjugated Goat anti-Rabbit IgG at 1:20,000 and TMB as the substrate. Appropriate specificity controls were run.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAnti-6X His tag® antibody (ab9108) is a rabbit polyclonal antibody and is validated for use in ChIP, ELISA, ICC\/IF, WB in human, mouse, rat samples.\u003cbr\u003e\nAnti-6X His tag® antibody (ab9108) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community.\u003cbr\u003e\nAbcam's high quality validation processes ensure Anti-6X His tag® antibody (ab9108) has high sensitivity and specificity.\u003cbr\u003e\nAnti-6X His tag® antibody (ab9108) has 25 independent reviews from customers.\u003cbr\u003e\n6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications (for example imaging and Flow cytometry). To enable specific detection of your tagged protein, Anti-6X His tag® antibody (ab9108) has been validated in ChIP, ELISA, ICC\/IF, WB 25 165\u003cbr\u003e\nAnti-6X His tag® antibody (ab9108) specifically detects 6X His tag® (UniProt ID: P26367; Molecular weight: 47kDa) and is sold in 100 µg selling sizes.\u003cbr\u003e\nThis antibody recognizes both C-terminal and N-terminal tags.\u003cbr\u003e\nAbcam recommended secondaries - Goat Anti-Rabbit HRP (\u003cbr\u003e\nab205718\u003cbr\u003e\n) and Goat Anti-Rabbit Alexa Fluor® 488 (\u003cbr\u003e\nab150077\u003cbr\u003e\n). Or search our wide range of secondary antibodies for use with your experiment.\u003cbr\u003e\nHIS-TAG® is a trademark of EMD Biosciences, Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nProteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.\u003cbr\u003e\nThe 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855689109673,"sku":"ab9108","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab9108","provider":"Iright","version":"1.0","type":"link"}