{"product_id":"abcam-ab91136","title":"Abcam, ab91136, Native human alpha 1 Antitrypsin protein (Active)","description":"\u003cp\u003eSize: 1mg\u003cbr\u003e\nNative human alpha 1 Antitrypsin protein (Active) is a Human Full Length A1AT\/SERPIN A1 protein, with \u0026gt;95% purity and suitable for western blot , SDS-PAGE and Functional studies. The predicted molecular weight of ab91136 native protein is 52 kDa. - Save time and ensure accurate results - use our A1AT\/SERPIN A1 protein - Optimal protein bioactivity and stability\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Native,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, WB, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inhibitory.,\u003cbr\u003e\nAccession:P01009,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in water,\u003cbr\u003e\nStorage buffer:pH: 6.5Constituents: 1.74% Sodium chloride, 0.492% Sodium phosphate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nThe Native human alpha 1 Antitrypsin protein (Active) ab91136 is prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 \u0026amp; 2 by FDA approved tests.\u003cbr\u003e\nEnsure the validity of your result using our native human alpha 1 Antitrypsin (A1AT\/SERPIN A1) protein as a control in SDS-PAGE.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nProtein Determination: Extinction Coefficient (ε) 0.1% at 280 nm, 1 cm pathway = 0.433\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAlpha 1 Antitrypsin also known as A1AT or alpha-1 proteinase inhibitor is a serine protease inhibitor with a molecular mass of about 52 kDa. This protein mainly expresses in the liver and found in high concentrations in the blood plasma. A1AT protects tissues from enzymes of inflammatory cells especially neutrophil elastase. Its expression level is regulated by the liver making it a significant player in maintaining tissue integrity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nA1AT regulates protease activity by forming complexes with target enzymes. It specifically inhibits neutrophil elastase a powerful enzyme capable of degrading elastin an important component of connective tissues. A1AT prevents excessive tissue damage during inflammation by maintaining a balance in protease activity within connective tissues across various organs.\u003cbr\u003e\nPathways\u003cbr\u003e\nAlpha 1 Antitrypsin functions within the proteolytic pathways involved in inflammatory response and tissue remodeling. A1AT is closely related to neutrophil elastase in these processes. It interacts with other protease inhibitors like alpha 2-macroglobulin reinforcing its protective role against enzymatic activity that can lead to tissue destruction under pathophysiological conditions.\u003cbr\u003e\nAlpha 1 Antitrypsin deficiency is a genetic condition that can cause chronic obstructive pulmonary disease (COPD) and liver cirrhosis. Deficient A1AT levels result in unregulated elastase activity leading to lung tissue damage and impaired liver function. Other proteins such as MMP-9 collaborate with elastase in exacerbating tissue damage illustrating how insufficient A1AT can significantly contribute to disease development.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843810447529,"sku":"ab91136","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab91136","provider":"Iright","version":"1.0","type":"link"}