{"product_id":"abcam-ab92312","title":"Abcam, ab92312, Anti-MLH1 antibody [EPR3894]","description":"\u003cp\u003eSize: 20µL \/ 100µL \/ 1mL\u003cbr\u003e\nAnti-MLH1 antibody [EPR3894] (ab92312) is a rabbit monoclonal antibody detecting MLH1 in  Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC\/IF . Suitable for  Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 80 publications - Trusted since 2010\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR3894,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nReacts with:Mouse, Rat, Human,\u003cbr\u003e\nApplications:WB, Flow Cyt (Intra), ICC\/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat is this antibody validated in?\u003cbr\u003e\nAnti-MLH1 antibody [EPR3894] (ab92312) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry\/immunofluorescence (ICC\/IF) in Human, Mouse, Rat samples.\u003cbr\u003e\nWhat is the molecular weight of MLH1?\u003cbr\u003e\nAnti-MLH1 [EPR3894] (ab92312) specifically detects a band for MLH1 (UniProt: P40692) at a molecular weight of 84kDa.\u003cbr\u003e\nTrusted by the scientific community\u003cbr\u003e\nAnti-MLH1 [EPR3894] (ab92312) was first used in a scientific publication in 2010 and has been cited over 80 times in peer-reviewed journals.\u003cbr\u003e\nTrial sizes available!\u003cbr\u003e\nTest your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.\u003cbr\u003e\nDiscover our selection of trial-size antibodies\u003cbr\u003e\nSpecificity confirmed\u003cbr\u003e\nThe specificity of Anti-MLH1 antibody [EPR3894] (ab92312) has been confirmed by Western blot testing in MLH1 Knockout HAP1 cell line,\u003cbr\u003e\nab276105\u003cbr\u003e\nOther related products\u003cbr\u003e\nWe have a range of other formats of antibody clone [EPR3894] also available for your convenience: ab92312, Alexa Fluor® 488 -\u003cbr\u003e\nab199237\u003cbr\u003e\n, Alexa Fluor® 647 -\u003cbr\u003e\nab199494\u003cbr\u003e\n, Carrier free -\u003cbr\u003e\nab214441\u003cbr\u003e\n, Alexa Fluor® 555 -\u003cbr\u003e\nab215303\u003cbr\u003e\n, PE -\u003cbr\u003e\nab303047\u003cbr\u003e\n, APC -\u003cbr\u003e\nab303048\u003cbr\u003e\n, HRP -\u003cbr\u003e\nab303049\u003cbr\u003e\n, Alkaline Phosphatase -\u003cbr\u003e\nab308849\u003cbr\u003e\n, Alexa Fluor® 594 -\u003cbr\u003e\nab310577\u003cbr\u003e\n, Alexa Fluor® 568 -\u003cbr\u003e\nab312589\u003cbr\u003e\n, Alexa Fluor® 750 -\u003cbr\u003e\nab321745\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nShipping conditions update: ambient shipping\u003cbr\u003e\nThis product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.\u003cbr\u003e\nWhy the change?\u003cbr\u003e\nIt’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.\u003cbr\u003e\nWhat you need to know\u003cbr\u003e\nAmbient shipments come clearly marked on the delivery note.\u003cbr\u003e\nNo ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.\u003cbr\u003e\nWarranty coverage remains fully valid, aligned with our validated shipping method.\u003cbr\u003e\nPlease store the product as per the datasheet instructions upon receipt.\u003cbr\u003e\nFind out more -\u003cbr\u003e\nhttps:\/\/www.abcam.com\/en-us\/support\/shipping-storage-support\/ambient-shipping\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nMLH1 also known as MutL homolog 1 is a protein involved in DNA mismatch repair an important mechanism for maintaining genetic stability. It has a molecular weight of approximately 87 kDa. This protein is expressed in various tissues but is most abundant in the colonic epithelium and endometrium. MLH1 acts mechanically by forming heterodimers with other proteins collaborating in correcting errors that occur during DNA replication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe function of MLH1 involves its role in the mismatch repair (MMR) system. It is part of a complex with PMS2 forming a heterodimer known as MutLα which is essential for the repair process. This complex scans newly synthesized DNA for mispaired bases and initiates repair preserving genomic integrity. The proper function of MLH1 and its interaction with PMS2 ensures that DNA replication errors do not accumulate and cause harmful mutations.\u003cbr\u003e\nPathways\u003cbr\u003e\nMLH1 operates within the mismatch repair pathway and interacts closely with MLH3 and PMS2 proteins. It plays a critical role in the recognition and repair of mismatched bases that occur during DNA replication particularly in the G2 phase of the cell cycle. Through its involvement in the mismatch repair pathway MLH1 is connected to cell cycle regulation and the DNA damage response pathway.\u003cbr\u003e\nMLH1 mutations are closely linked to Lynch syndrome and sporadic colorectal cancer. Lynch syndrome a hereditary condition significantly raises the risk of colorectal cancer and other cancers due to defective DNA mismatch repair. MLH1 mutations often lead to the loss of MLH1 protein expression particularly observed in MLH1 IHC staining. Additionally in colorectal cancer the MLH1 protein may interact with APC and TP53 playing a role in cancer progression and tumorigenesis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844032516265,"sku":"ab92312","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab92312","provider":"Iright","version":"1.0","type":"link"}