{"product_id":"abcam-ab93684","title":"Abcam, ab93684, Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)","description":"\u003cp\u003eSize: 125mL \/ 250mL\u003cbr\u003e\nAntigen retrieval buffer (100X Tris-EDTA, pH 9.0) (ab93684) is used in heat-induced antigen retrieval (HIER) to enhance staining on formalin-fixed, paraffin-embedded tissue sections with our primary antibody range during IHC. - Provided in 100X solution for easy dilution preparation - Available in different sizes to fit your experimental needs - Compatible with our Lab essentials staining workflow offering - Proven performance: cited in over 85 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nApplications:IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 8.7 - 9.1Constituents: 5% Tris, 5% Tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAntigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) enables target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require Tris-EDTA buffer (pH 9.0) pretreatment.\u003cbr\u003e\nThis product contains detergent for emulsification of the paraffin.\u003cbr\u003e\n1X Dilution:\u003cbr\u003e\nThe 100X stock solution should be diluted 100-fold with distilled water before use.\u003cbr\u003e\nProtocol:\u003cbr\u003e\nPlace paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.\u003cbr\u003e\nWash sections with hot tap water for 1 minute.\u003cbr\u003e\nWash sections in buffer for 2x3 minutes.\u003cbr\u003e\nContinue with IHC protocol\u003cbr\u003e\nOther kits and reagents for IHC\u003cbr\u003e\nOther antigen retrieval buffers include: Citrate buffer pH 6.0\u003cbr\u003e\nab93678\u003cbr\u003e\n, EDTA buffer pH 8.0\u003cbr\u003e\nab93680\u003cbr\u003e\n, Tris buffer pH 10.0\u003cbr\u003e\nab93682\u003cbr\u003e\n, or see the full list of\u003cbr\u003e\nantigen retrieval buffers and enzymes\u003cbr\u003e\nFind more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the\u003cbr\u003e\nIHC kits and reagents guide\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847571067049,"sku":"ab93684","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab93684","provider":"Iright","version":"1.0","type":"link"}