{"product_id":"abcam-ab95310","title":"Abcam, ab95310, Recombinant human PAK2 protein","description":"\u003cp\u003eSize: 10µg\u003cbr\u003e\nRecombinant human PAK2 protein is a Human Fragment protein, in the 227 to 524 aa range, expressed in Escherichia coli, with \u0026gt;90%, suitable for FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;90% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:86 nmoles\/min\/μg Analysis of enzymatic activity: 1. Different concentrations of PAK2 were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER\/THR 20) at room temperature for 1 hour. 2. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT. 3. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm filters.,\u003cbr\u003e\nAccession:Q13177,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.395% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46844003319977,"sku":"ab95310","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab95310","provider":"Iright","version":"1.0","type":"link"}