{"product_id":"abcam-ab99995","title":"Abcam, ab99995, Human C Reactive Protein ELISA Kit (CRP)","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman C Reactive Protein ELISA Kit (CRP) is a sandwich ELISA designed to quantify Human C Reactive Protein (CRP) with a sensitivity of 2 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 34.29 - 25000 pg\/mL - Cited in over 30 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:\u0026lt; 2 pg\/mL,\u003cbr\u003e\nRange:34.29 - 25000 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman C Reactive Protein ELISA Kit (CRP) ab99995 is a sandwich ELISA to measure Human C Reactive Protein (CRP) in serum, plasma, cell culture supernatant with a sensitivity of 2 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for Human CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman C Reactive Protein ELISA Kit (CRP) ab99995 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature\u003cbr\u003e\n2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Wash and add prepared streptavidin solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Development Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450nm immediately.\u003cbr\u003e\nGet results in 90 minutes with our SimpleStep ELISA\u003cbr\u003e\nHuman C Reactive Protein ELISA Kit (\u003cbr\u003e\nab181416\u003cbr\u003e\nOptimization may be required with urine samples.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nC-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.\u003cbr\u003e\nPathways\u003cbr\u003e\nCRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.\u003cbr\u003e\nElevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843608924329,"sku":"ab99995","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/abcam-ab99995","provider":"Iright","version":"1.0","type":"link"}