Agilent, 102720-100, Seahorse XF Palmitate-BSA FAO Substrate

Seahorse XF Palmitate-BSA FAO Substrate. Each product includes three (3) vials of Seahorse XF Palmitate-BSA (2ml/each) and three (3) vials of Seahorse XF BSA control (2mL/each), and is sufficient for 3 full plate assays. For use with Seahorse XFe/XF96 and XF/XF24 analyzers.

Specifications:
Assay Wells: 3 x 24-well or 96-well assays
Assays per Kit: 3
For Use With: Seahorse XF Pro AnalyzerSeahorse XF96 AnalyzerSeahorse XFe24 AnalyzerSeahorse XFe96 Analyzer
Kit Contents: Palmitate-BSA: 3 x 2 mL vials, 1 mM Palmitate conjugated to 0.17 mM BSA (free fatty acid concentration listed on box for each lot). BSA Control - 3 x 2 mL vials, 0.17 mM BSA solution.
Shipping Condition: Ambient temperature in an insulated container
Storage Temp Conditions: Freezer storage required, -20°C

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This substrate provides a convenient, ready-to-use solution designed specifically to support fatty acid oxidation (FAO) analyses in live cell metabolic assays. Each vial contains palmitate conjugated to BSA, enabling efficient delivery and uptake of long-chain fatty acids by cells. It is ideal for experiments aimed at investigating mitochondrial function, energy metabolism, or metabolic flexibility in various cell types, including primary, stem, cancer, and immune cells.

Researchers can utilize this reagent in applications such as studying substrate preference, assessing metabolic shifts in disease models, or screening compounds that impact FAO pathways. It is optimized for use with Agilent Seahorse XF Analyzers, ensuring robust and reproducible oxygen consumption rate (OCR) measurements during metabolic flux analyses. The formulation allows for straightforward integration with established assay protocols, particularly with the Seahorse XF Cell Mito Stress Test or specialized FAO assay kits.

For best results, this substrate should be prepared and applied according to detailed Seahorse assay guidelines. It is also compatible with a range of XF microplates and stress test media formulations. This product streamlines experimental workflows, delivering reliable insights into cellular lipid metabolism and supporting advanced metabolic research applications.