{"product_id":"biolegend-100228","title":"Biolegend, 100228, Brilliant Violet 421™ anti-mouse CD3 Antibody, 50μg","description":"\u003cp\u003eCD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: γδTCR-positive T-T hybridoma D1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunocytochemistry using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Diamantopoulou Z, et al. 2022. Nature. 607:156. PubMed Sanmarco LM, et al. 2022. Nature. 611:801. PubMed Liu H, et al. 2023. Breast Cancer Res. 25:43. PubMed Bhaskar A, et al. 2023. iScience. 26:106644. PubMed Kalinina A, et al. 2021. STAR Protoc. 2:100368. PubMed Schädlich IS, et al. 2022. iScience. 25:104470. PubMed Mills EL, et al. 2022. Cell Metab. 34:140. PubMed Kiani Shabestari S, et al. 2022. Cell Rep. 39:110961. PubMed Jin SM, et al. 2023. Nat Nanotechnol. :. PubMed Oh S, et al. 2023. Nat Biotechnol. :. PubMed John S, et al. 2020. Sci Rep. 10:11377. PubMed Chukkapalli S, et al. 2015. Pathog Dis. 73:9. PubMed Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed Chen J, et al. 2022. Nat Commun. 13:6759. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed Almshayakhchi R, et al. 2021. Front Oncol. 11:636977. PubMed Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed Bertocchi A, et al. 2021. Cancer Cell. 39(5):708-724.e11. PubMed Chao CC, et al. 2020. Cell. 179(7):1483-1498.e22.. PubMed Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed Trenevska I, et al. 2021. PLoS One. 16:e0249967. PubMed Emgård J, et al. 2018. Immunity. 143:419. PubMed Glaser AK, et al. 2019. Nat Commun. 10:2781. PubMed Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed Cameron G, et al. 2015. J Immunol. 195: 4604 - 4614. PubMed Telford W, et al. 2017. Cytometry A. 91:314. PubMed Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed Chukkapalli S, et al. 2015. PLoS One. 10: 0143291. PubMed Jin C, et al. 2019. Cell. 176:998. PubMed Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed Hu Z, et al. 2020. PLoS One. 15:e0228339. PubMed Zhu D, et al. 2017. Stem Cell Res Ther. 0.511805556. PubMed Bristow CL, et al. 2021. Front Oncol. 11:739080. PubMed Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed Royzman D, et al. 2019. Front Immunol. 10:633. PubMed Heil J, et al. 2021. Nat Commun. 12:6963. PubMed Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed\u003cbr\u003e\nRRID: AB_10900227 (BioLegend Cat. No. 100227) AB_2562553 (BioLegend Cat. No. 100228)\u003cbr\u003e\nStructure: Ig superfamily, CD3\/TCR, 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: Antigen recognition, TCR signal transduction, T cell activation\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12502\u003cbr\u003e\nUniProt: View information about CD3 on UniProt.org\u003cbr\u003e\nClone: 17A2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T cell antigen receptor complex, T3\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860825428137,"sku":"100228","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-100228","provider":"Iright","version":"1.0","type":"link"}