{"product_id":"biolegend-100329","title":"Biolegend, 100329, APC\/Cyanine7 anti-mouse CD3ε Antibody, 25μg","description":"\u003cp\u003eCD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α\/β or γ\/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide\/MHC antigen complex.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with APC\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt;0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin \u0026lt;0.01 EU\/µg).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block) Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ) Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB) Payer E, et al. 1991. J. Immunol. 146:2536. (IF) Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD) Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ) Henrickson M, et al. 1995. Transplantation 60:828. (Deplete) Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete) Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete) Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ) Ko SY, et al. 2005. J. Immunol. 175:3309. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F) Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F) Wang W, et al. 2007. J. Immunol. 178:4885. (Activ) Xiao S, et al. 2007. J. Exp. Med. 204:1691. Chappaz S, et al. 2007. Blood doi:10.1182\/blood-2007-02-074245. (FC) PubMed. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed Guo Y, et al. 2008. Blood 112:480. PubMed Kenna TJ, et al. 2008. Blood 111:2091. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed Schulteis RD, et al. 2008. Blood 295:G273. PubMed Qi Q, et al. 2009. Blood 114:564. PubMed Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed Yan J, et al. 2014. Vaccine. 32:2833. PubMed Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)\u003cbr\u003e\nProduct Citations: Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038\/s41598-017-10135-1. PubMed Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed Wiedemann SJ, et al. 2022. Cell Metab. 34:991. PubMed Hao H, et al. 2023. Nat Biomed Eng. . PubMed Zhou L, et al. 2022. PLoS Negl Trop Dis. 16:e0010851. PubMed Ge Y, et al. 2022. iScience. 25:105437. PubMed Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed Issa N, et al. 2023. Leukemia. :. PubMed Roberts JL, et al. 2022. Bone. 157:116310. PubMed Leonardi I, et al. 2022. Cell. 185:831. PubMed Potempa M, et al. 2022. J Immunol. 208:1742. PubMed Miao L, et al. 2020. Theranostics. 0.7625. PubMed Zhou J, et al. 2019. Immunity. 50:403. PubMed Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed Meng J, et al. 2011. Biol Reprod. 85:254. PubMed Wei H, et al. 2021. Malar J. 20:89. PubMed Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed Chikina AS, et al. 2020. Cell. 183:411. PubMed Rao S, et al. 2017. Cell. 168(3):503-516.e12. PubMed Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed Yang X, et al. 2019. Oncotarget. 10:4180. PubMed Dai B, et al. 2022. Theranostics. 12:7603. PubMed Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed Uchil PD et al. 2018. Cell host \u0026amp; microbe. 25(1):87-100 . PubMed Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed Fantauzzi MF, et al. 2021. ERJ Open Res. 7: . PubMed Kong YW, et al. 2020. Nat Commun. 3.322222222. PubMed Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed Noah AC, et al. 2020. J Appl Physiol (1985). 473:128. PubMed Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed Ekeke CN, et al. 2020. J Thorac Cardiovasc Surg. S0022-5223:33337. PubMed Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed Qi S et al. 2016. eLife. 5 pii: e14756. PubMed Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed Shoji M, et al. 2012. Biochem Biophys Res Commun. 425:89. PubMed Kim S, et al. 2021. Elife. 10:. PubMed Shen M, et al. 2022. Nat Cancer. 3:60. PubMed Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed Yang S, et al. 2022. J Exp Med. 219:. PubMed Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed Kim M, et al. 2018. Immunity. 49:151. PubMed Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed Fujii T, et al. 2021. Bone Res. 9:4. PubMed Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed Celik H, et al. 2018. Cancer Cell. 34:741. PubMed Barbet G, et al. 2018. Immunity. 48:584. PubMed Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed Mah-Som AY, et al. 2021. Cell Reports. 35(9):109209. PubMed Brown ZJ, et al. 2018. Cell Death Dis. 0.805555556. PubMed Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed Rivera CA, et al. 2022. Immunity. 55:129. PubMed Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed Campisi L, et al. 2016. Nat Immunol. 10.1038\/ni.3512. PubMed Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed Tsai S, et al. 2018. Cell Metab. 28:922. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed\u003cbr\u003e\nRRID: AB_1877171 (BioLegend Cat. No. 100329) AB_1877170 (BioLegend Cat. No. 100330)\u003cbr\u003e\nStructure: Ig superfamily, forms CD3\/TCR complex with CD3δ, γ and ζ subunits and TCR (α\/β and γ\/δ), 20 kD\u003cbr\u003e\nDistribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells\u003cbr\u003e\nFunction: TCR signal transduction, T cell activation, antigen recognition\u003cbr\u003e\nLigand\/Receptor: Peptide antigen\/MHC-complex\u003cbr\u003e\nCell Type: NKT cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: CD Molecules, TCRs\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.\u003cbr\u003e\nGene ID: 12501\u003cbr\u003e\nUniProt: View information about CD3epsilon on UniProt.org\u003cbr\u003e\nClone: 145-2C11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD3ε, T3, CD3\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860803440809,"sku":"100329","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-100329","provider":"Iright","version":"1.0","type":"link"}