{"product_id":"biolegend-100606","title":"Biolegend, 100606, FITC anti-mouse CD5 Antibody, 500μg","description":"\u003cp\u003eCD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α\/β T cells express high levels of CD5, very few γ\/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370.\u003cbr\u003e\nProduct Citations: Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed Collin R, et al. 2020. J Immunol. 205:133. PubMed Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed Alotaibi F, et al. 2021. Front Immunol. 11:584937. PubMed Lutes LK, et al. 2021. eLife. 10:00. PubMed Ganeshan K et al. 2019. Cell. 177(2):399-413 . PubMed Solanki A, et al. 2018. Development. 145. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Mues M, et al. 2019. Cell Rep. 27:631. PubMed Kruta M, et al. 2021. Cell Stem Cell. :. PubMed Ishikawa E, et al. 2016. Nat Commun. 7: 12756. PubMed Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed Nowlan B, et al. 2019. Haematologica. 105:71. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Vacca F, et al. 2020. eLife. 9:e54017.. PubMed Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed Yin S et al. 2019. Cancer cell. 35(2):283-296 . PubMed Chen C, et al. 2020. Cell Rep. 2136:30. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed Li K, et al. 2020. Nat Commun. 0.795138889. PubMed Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed Campbell J, et al. 2010. J Immunol. 185:28:00. PubMed Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed Gu Z, et al. 2021. Nat Genet. 53:672. PubMed\u003cbr\u003e\nRRID: AB_312734 (BioLegend Cat. No. 100605) AB_312735 (BioLegend Cat. No. 100606)\u003cbr\u003e\nStructure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD\u003cbr\u003e\nDistribution: Thymocytes, T cells, B-1 cells\u003cbr\u003e\nFunction: Negative regulator of T-B cell interaction\u003cbr\u003e\nLigand\/Receptor: CD72, gp35-37\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.\u003cbr\u003e\nGene ID: 12507\u003cbr\u003e\nUniProt: View information about CD5 on UniProt.org\u003cbr\u003e\nClone: 53-7.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-1, Ly-1, T1, Tp67, Ly-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862440235177,"sku":"100606","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-100606","provider":"Iright","version":"1.0","type":"link"}