{"product_id":"biolegend-100607","title":"Biolegend, 100607, PE anti-mouse CD5 Antibody, 50μg","description":"\u003cp\u003eCD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α\/β T cells express high levels of CD5, very few γ\/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370.\u003cbr\u003e\nProduct Citations: Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed Liu J, et al. 2021. Front Immunol. 12:747730. PubMed Erickson JJ, et al. 2022. Nature. 606:769. PubMed Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed Solanki A, et al. 2018. Development. 145. PubMed Webster P, et al. 2018. Nat Commun. 9:2649. PubMed Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed Kruta M, et al. 2021. Cell Stem Cell. :. PubMed LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed Xu M, et al. 2013. J Immunol. 190:5436. PubMed Orlanski S, et al. 2016. Proc Natl Acad Sci U S A. 113: 5018-5023. PubMed Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed Hirose T, et al. 2017. PLoS One. 12(6):e0178765. PubMed Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed Snapper Q 2013. J Immunol. 190:1048. PubMed\u003cbr\u003e\nRRID: AB_312736 (BioLegend Cat. No. 100607) AB_312737 (BioLegend Cat. No. 100608)\u003cbr\u003e\nStructure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD\u003cbr\u003e\nDistribution: Thymocytes, T cells, B-1 cells\u003cbr\u003e\nFunction: Negative regulator of T-B cell interaction\u003cbr\u003e\nLigand\/Receptor: CD72, gp35-37\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.\u003cbr\u003e\nGene ID: 12507\u003cbr\u003e\nUniProt: View information about CD5 on UniProt.org\u003cbr\u003e\nClone: 53-7.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-1, Ly-1, T1, Tp67, Ly-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862441250985,"sku":"100607","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-100607","provider":"Iright","version":"1.0","type":"link"}