{"product_id":"biolegend-100618","title":"Biolegend, 100618, Brilliant Violet 421™ anti-mouse CD5 Antibody, 500microl","description":"\u003cp\u003eCD5 is a 67 kD protein, also known as Lyt-1, Ly-1, T1, Tp67, or Ly-12. It is a member of the scavenger receptor cysteine-rich protein superfamily (SRCR) and primarily expressed on thymocytes, T cells, and B-1 cells. Although mature α\/β T cells express high levels of CD5, very few γ\/δ T cells express this antigen. The interaction of CD5 with CD72, gp35-37, TCR, or BCR is involved in T and B cell activation.\u003cbr\u003e\n500microl\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse thymus or spleen\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.2 mg\/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.03 µg per million cells in 100 µl volume. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, and immunohistochemistry2 of acetone-fixed frozen tissue sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP) Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (FC, IHC) Bourdeau A, et al. 2007. Blood doi:10.1182\/blood-2006-08-044370.\u003cbr\u003e\nProduct Citations: Schneider C, et al. 2018. Cell. 174:271. PubMed Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed Sugita Y, et al. 2021. Oncol Rep. 45:. PubMed Parigger T, et al. 2021. Int J Mol Sci. 22:. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed\u003cbr\u003e\nRRID: AB_2562173 (BioLegend Cat. No. 100617) AB_2565430 (BioLegend Cat. No. 100629) AB_2562174 (BioLegend Cat. No. 100618)\u003cbr\u003e\nStructure: Member of the scavenger receptor cysteine-rich protein superfamily (SRCR), 67 kD\u003cbr\u003e\nDistribution: Thymocytes, T cells, B-1 cells\u003cbr\u003e\nFunction: Negative regulator of T-B cell interaction\u003cbr\u003e\nLigand\/Receptor: CD72, gp35-37\u003cbr\u003e\nCell Type: B cells, T cells, Thymocytes\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kipps TJ. 1988. Adv. Immunol. 47:117. 3. Antin JH, et al. 1985. J. Immunol. 136:505. 4. Tarakhovsky A, et al. 1995. Science 269:535.\u003cbr\u003e\nGene ID: 12507\u003cbr\u003e\nUniProt: View information about CD5 on UniProt.org\u003cbr\u003e\nClone: 53-7.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lyt-1, Ly-1, T1, Tp67, Ly-12\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862441939113,"sku":"100618","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-100618","provider":"Iright","version":"1.0","type":"link"}