{"product_id":"biolegend-102106","title":"Biolegend, 102106, PE anti-mouse CD28 Antibody, 200μg","description":"\u003cp\u003eCD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.\u003cbr\u003e\n200μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Syrian Hamster\u003cbr\u003e\nImmunogen: C57BL\/6 mouse T-cell lymphoma EL-4\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim) Cibotti R, et al. 1997. Immunity 6:245. (Costim) Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) Nishio M, et al. 1996. J. Immunol. 157:4347. (Block) Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim) Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim) Wang W, et al. 2007. J. Immunol. 178:4885. (Costim) Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim) Perchonock CE, et al. 2007. J. Immunol. 179:1768. Barbi J, et al. 2007. Blood 110:2215. Milpied P, et al. 2011. Blood 118:2993. PubMed Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed Li CR, et al. 2014. J Immunol. 192:1425. PubMed Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed\u003cbr\u003e\nProduct Citations: Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Xie A et al. 2017. Endocrinology. 158(10):3140-3151 . PubMed Qi Z, et al. 2022. Nat Commun. 13:182. PubMed Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed Sasayama S, et al. 2018. Int J Mol Sci. 19:. PubMed Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed\u003cbr\u003e\nRRID: AB_312870 (BioLegend Cat. No. 102105) AB_312871 (BioLegend Cat. No. 102106)\u003cbr\u003e\nDisclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.\u003cbr\u003e\nStructure: Ig superfamily, 44 kD\u003cbr\u003e\nDistribution: Thymocytes, CD4+, CD8+ peripheral T cells, NK cells\u003cbr\u003e\nFunction: Costimulates T and NK cells\u003cbr\u003e\nLigand\/Receptor: CD80 (B7-1), CD86 (B7-2)\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.\u003cbr\u003e\nGene ID: 12487\u003cbr\u003e\nUniProt: View information about CD28 on UniProt.org\u003cbr\u003e\nClone: 37.51\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tp44, T44\u003cbr\u003e\nIsotype: Syrian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862380859561,"sku":"102106","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-102106","provider":"Iright","version":"1.0","type":"link"}