{"product_id":"biolegend-102127","title":"Biolegend, 102127, Brilliant Violet 421™ anti-mouse CD28 Antibody, 50μg","description":"\u003cp\u003eCD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Syrian Hamster\u003cbr\u003e\nImmunogen: C57BL\/6 mouse T-cell lymphoma EL-4\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450\/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim) Cibotti R, et al. 1997. Immunity 6:245. (Costim) Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) Nishio M, et al. 1996. J. Immunol. 157:4347. (Block) Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim) Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim) Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim) Wang W, et al. 2007. J. Immunol. 178:4885. (Costim) Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim) Perchonock CE, et al. 2007. J. Immunol. 179:1768. Barbi J, et al. 2007. Blood 110:2215. Milpied P, et al. 2011. Blood 118:2993. PubMed Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed Li CR, et al. 2014. J Immunol. 192:1425. PubMed Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed\u003cbr\u003e\nProduct Citations: Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed Marangoni F, et al. 2021. Cell. . PubMed Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed\u003cbr\u003e\nRRID: AB_2650628 (BioLegend Cat. No. 102127)\u003cbr\u003e\nDisclaimer: This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.\u003cbr\u003e\nStructure: Ig superfamily, 44 kD\u003cbr\u003e\nDistribution: Thymocytes, CD4+, CD8+ peripheral T cells, NK cells\u003cbr\u003e\nFunction: Costimulates T and NK cells\u003cbr\u003e\nLigand\/Receptor: CD80 (B7-1), CD86 (B7-2)\u003cbr\u003e\nCell Type: NK cells, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Costimulatory Molecules, Immunology\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.\u003cbr\u003e\nGene ID: 12487\u003cbr\u003e\nUniProt: View information about CD28 on UniProt.org\u003cbr\u003e\nClone: 37.51\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tp44, T44\u003cbr\u003e\nIsotype: Syrian Hamster IgG\u003cbr\u003e\nQ: What is the F\/P ratio range of our BV421™ format antibody reagents?\u003cbr\u003e\nA: It is lot-specific. On average it ranges between 2-4.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862381940905,"sku":"102127","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-102127","provider":"Iright","version":"1.0","type":"link"}