{"product_id":"biolegend-102401","title":"Biolegend, 102401, Purified anti-mouse CD31 Antibody, 50μg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed Wei G, et al. 2023. Pharmaceutics. 15:. PubMed Sugita Y, et al. 2023. PLoS One. 18:e0279634. PubMed Shin JW, et al. 2022. Cell Rep Methods. 2:100338. PubMed Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed Hata E, et al. 2016. Int Immunol. 28: 283 - 292. PubMed Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed Greineder C, et al. 2013. PLoS One. 14:80110. PubMed Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed Dubrot J, et al. 2018. Life Sci Alliance. 1:e201800164. PubMed Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed Gong F, et al. 2020. Nat Commun. 3.036111111. PubMed Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed Stefanescu C, et al. 2021. Front Oncol. 11:765151. PubMed Nessler I, et al. 2020. Cancer Res. 80:1268. PubMed Rodionov RN, et al. 2022. Sci Rep. 12:9381. PubMed Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed Molica F,et al. 2017. Sci Rep.. 10.1038\/s41598-017-14130-4. PubMed Lim PJ, et al. 2019. Nat Metab. 0.402083333. PubMed Sun W, et al. 2021. Mol Ther. 29:1312. PubMed Bertrand JA, et al. 2020. Invest Ophthalmol Vis Sci. 61:41:00. PubMed Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed Shin J, et al. 2018. Diabetes. 67:1068. PubMed Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed Yan SLS, et al. 2021. STAR Protoc. 2:100498. PubMed Bhatnagar S, et al. 2018. Mol Pharm. 3. PubMed Kimishima Y, et al. 2021. Nat Commun. 12:6177. PubMed González LA, et al. 2021. Front Immunol. 12:638917. PubMed Alexandrushkina N, et al. 2020. Int J Mol Sci. 21:00. PubMed Khoa LTP, et al. 2020. STAR Protoc. 1:100136. PubMed Wang W, et al. 2018. J Vasc Surg. 68:1538. PubMed Taniguchi Y, et al. 2020. PLoS One. 15:e0228203. PubMed Tamburini BAJ, et al. 2019. Front Immunol. 10:1036. PubMed Subramanian BC, et al. 2020. J Cell Biol. 219:. PubMed Khera E, et al. 2021. Neoplasia. 1.104166667. PubMed Stepanova V, et al. 2016. J Biol Chem. 291: 15029 - 15045. PubMed Blaskovic S, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L606:318. PubMed Eslani M, et al. 2018. Stem Cells. 36:775. PubMed Fujimura N, et al. 2016. J Vasc Surg. 64: 46-54. PubMed Xiong S, et al. 2020. J Clin Invest. 130:3684. PubMed Lopes N, et al. 2017. EMBO Mol Med. 0.954861111. PubMed Cilliers C, et al. 2018. Cancer Res. 78:758. PubMed Murray CW, et al. 2022. Nat Commun. 13:1090. PubMed\u003cbr\u003e\nRRID: AB_312896 (BioLegend Cat. No. 102401) AB_312897 (BioLegend Cat. No. 102402)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862383874217,"sku":"102401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-102401","provider":"Iright","version":"1.0","type":"link"}