{"product_id":"biolegend-102406","title":"Biolegend, 102406, FITC anti-mouse CD31 Antibody, 500μg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed Wang C, et al. 2023. iScience. 26:106272. PubMed Wang Y, et al. 2023. Cell Prolif. 56:e13447. PubMed Hattori Y, et al. 2022. J Neurosci. 42:362. PubMed Charoensaensuk V, et al. 2022. Antioxidants (Basel). 11:. PubMed Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed Wang W, et al. 2022. Adv Sci (Weinh). 9:e2105208. PubMed Leinroth AP, et al. 2022. Cell Rep. 39:110785. PubMed Tsumita T, et al. 2022. Int J Cancer. 151:944. PubMed Ge Y, et al. 2022. J Exp Med. 219: . PubMed Ge Y, et al. 2022. iScience. 25:105437. PubMed Ge Y, et al. 2022. STAR Protoc. 4:101936. PubMed Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed Urban SK, et al. 2020. Liver Int. 40:3103. PubMed Sullivan D, Watson R, Muller W 2016. Am J Physiol Heart Circ Physiol. 311: H621 - H632. PubMed Yoshida Y, et al. 2015. J Mol Cell Cardiol. 85: 183-198. PubMed Gao Q, et al. 2019. J Exp Med. 216:688. PubMed Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed Li X, et al. 2022. Nat Commun. 13:2794. PubMed Stubelius A, et al. 2017. Immunobiology. 10.1016\/j.imbio.2017.05.007. PubMed Royzman D, et al. 2022. Front Immunol. 13:1012647. PubMed Menzel L, et al. 2022. STAR Protoc. 3:101267. PubMed Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed Charoensaensuk V, et al. 2021. Cells. 10:. PubMed Diebold LP, et al. 2019. Nat Metab. 1:158. PubMed Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed Carrick FR, et al. 2021. Life (Basel). 11:. PubMed Wen A, et al. 2012. J Vis Exp. 69: 4352. PubMed Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed Scur M, et al. 2022. Nat Commun. 13:7272. PubMed Yong T, et al. 2019. Nat Commun. 10:3838. PubMed Kotla S, et al. 2019. JCI Insight. 4:9. PubMed Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed Li CM, et al. 2020. Cell Reports. 33(13):108566. PubMed Yip HYK, et al. 2018. PLoS One. 3. PubMed Long JT, et al. 2022. Elife. 11:. PubMed Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed Sakai H, et al. 2022. iScience. 25:103928. PubMed Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed Zhao Q, et al. 2020. Cancer Res. 80:5531. PubMed Kasuga A, et al. 2020. Cancer Sci. . PubMed Usui-Ouchi A, et al. 2020. Proc Natl Acad Sci U S A. 28297:117. PubMed Hu J, et al. 2022. Stem Cell Res Ther. 13:349. PubMed\u003cbr\u003e\nRRID: AB_312900 (BioLegend Cat. No. 102405) AB_312901 (BioLegend Cat. No. 102406)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862382858409,"sku":"102406","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-102406","provider":"Iright","version":"1.0","type":"link"}