{"product_id":"biolegend-102418","title":"Biolegend, 102418, PE\/Cyanine7 anti-mouse CD31 Antibody, 100μg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed Hong X, et al. 2022. Cell Stem Cell. 29:1298. PubMed Mohamed GA, et al. 2023. Breast Cancer Res. 25:23. PubMed Cheong LY, et al. 2023. Nat Commun. 14:1213. PubMed Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed Moiseeva V, et al. 2023. Nature. 613:169. PubMed Varshney R, et al. 2023. iScience. 26:105750. PubMed Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed Yagishita Y, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed Weeden C, et al. 2017. PLoS Biol. 10.1371\/journal.pbio.2000731. PubMed Smith NR, et al. 2017. Cell Mol Gastroenterol Hepatol. 0.395138889. PubMed García-Prat L, et al. 2020. Nat Cell Biol. 22:1307. PubMed Hu X, et al. 2016. Nat Commun. 7:13095. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Luo R, et al. 2021. Theranostics. 11:117. PubMed Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed DellOrso S, et al. 2021. STAR Protocols. 2(2):100451. PubMed Best SA, et al. 2018. Cell Metab. 27:935. PubMed Minatoguchi S, et al. 2022. Sci Rep. 12:5389. PubMed Han YH, et al. 2021. Science. 373:. PubMed Campanario S, et al. 2021. Front Cell Dev Biol. 8:620409. PubMed Solanas G, et al. 2017. Cell. 170:678. PubMed Plein A, et al. 2018. Nature. 562:223. PubMed Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed Kumar B, et al. 2020. Haematologica. Online ahead of print:. PubMed Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Reddy A, et al. 2020. Cell. 183:62. PubMed Halpern KB, et al. 2018. Nat Biotechnol. 36:962. PubMed Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed Ni C, et al. 2022. Nat Commun. 13:5919. PubMed Pezoldt J, et al. 2022. Nat Commun. 13:7227. PubMed Zink J, et al. 2021. EMBO Rep. 22:e48961. PubMed Gordon E, et al. 2010. Development. 137:3899. PubMed Berg AL, et al. 2022. Cancers (Basel). 14:. PubMed Su Y, et al. 2020. Cell Death Differ. 949:27. PubMed Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed Linnerbauer M, et al. 2022. JCI Insight. 7:. PubMed Ito K, et al. 2014. Proc Natl Acad Sci U S A. 111:3080. PubMed Merrell AJ, et al. 2021. Hepatology. 74:444. PubMed Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed Schwörer S, et al. 2016. Nature. 540:428-432. PubMed Stock M, et al. 2015. Osteoarthritis Cartilage. 23: 1221-1225. PubMed Cheng HW, et al. 2022. Nat Commun. 13:2027. PubMed Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed El Agha E, et al. 2017. Cell Stem Cell. 1.014583333. PubMed Yousef H, et al. 2019. Nat Med. 25:988. PubMed Ben-Moshe S, et al. 2019. Nat Metab. 1:899. PubMed Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed Lu Z, et al. 2021. J Clin Invest. 131:. PubMed Mesnieres M, et al. 2021. Cell Reports. 36(8):109618. PubMed Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed Geng Y, et al. 2022. Nat Commun. 13:7114. PubMed Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed Yoldi G, et al. 2016. Cancer Res . 76: 5857 - 5869. PubMed Arora S, et al. 2021. Med (N Y). 2:938. PubMed Dong H, et al. 2022. Nat Metab. 4:90. PubMed Nakao M, et al. 2021. Sci Rep. 11:18384. PubMed Kimmel JC, et al. 2021. Cell Reports. 35(4):109046. PubMed Kabir AU, et al. 2018. JCI Insight. 3. PubMed Cui Y, et al. 2020. Eur J Pharmacol. 881:173233. PubMed Zhu Q, et al. 2021. J Am Heart Assoc. 10:e023491. PubMed\u003cbr\u003e\nRRID: AB_830756 (BioLegend Cat. No. 102417) AB_830757 (BioLegend Cat. No. 102418)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862383743145,"sku":"102418","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-102418","provider":"Iright","version":"1.0","type":"link"}