{"product_id":"biolegend-102427","title":"Biolegend, 102427, Brilliant Violet 605™ anti-mouse CD31 Antibody, 50μg","description":"\u003cp\u003eCD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α v β 3 integrin, CD51\/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: C3H\/HeJ mouse hematopoietic progenitor cell line 3\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610\/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin \u0026lt; 0.1 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Baldwin HS, et al. 1994. Development 120:2539. (IP, Block) DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) Rosenblum WI, et al. 1996. Stroke 27:709. (Block) Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block) Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC) Wiewrodt R, et al. 2002. Blood 99:912. (IHC) McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed Chacko AM, et al. 2012. PLoS One 7:e34958. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed\u003cbr\u003e\nProduct Citations: Korsunsky I, et al. 2022. Med. 3:481. PubMed Wang C, et al. 2023. iScience. 26:106272. PubMed Fowler JWM, et al. 2022. Elife. 11: . PubMed Li B, et al. 2023. Nat Commun. 14:489. PubMed AR P, et al. 2016. Circ Res. 118: 400-409. PubMed Nicolás-Ávila JÁ, et al. 2021. STAR Protoc. 2:100286. PubMed Mariani SA, et al. 2019. Immunity. 50:1439. PubMed Wang C, et al. 2021. Cell Rep. 37:110021. PubMed Ibrahim A, et al. 2020. Cell Metabolism. 32(2):309-319.e7. PubMed Benechet AP, et al. 2019. Nature. 574:200. PubMed Fu H, et al. 2020. Front Immunol. 11:595813. PubMed Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed De Simone G, et al. 2021. Immunity. :. PubMed Vink CS, et al. 2020. Cell Rep. 31:107627. PubMed Blriot C, et al. 2021. Immunity. :. PubMed Clark JT, et al. 2021. eLife. 10:00. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed Matthews O, et al. 2020. EBioMedicine. 62:103092. PubMed Gangoso E, et al. 2021. Cell. 184:2454. PubMed Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed Thomson CA, et al. 2018. J Immunol. 201:215. PubMed Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed\u003cbr\u003e\nRRID: AB_2563982 (BioLegend Cat. No. 102427)\u003cbr\u003e\nStructure: Ig superfamily, 130-140 kD\u003cbr\u003e\nDistribution: Endothelial cells, platelets, granulocytes, monocytes\/macrophages, dendritic cells, T and B cell subsets\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: CD38, αV\/β3 integrin\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells\u003cbr\u003e\nBiology Area: Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.\u003cbr\u003e\nGene ID: 18613\u003cbr\u003e\nUniProt: View information about CD31 on UniProt.org\u003cbr\u003e\nClone: 390\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PECAM-1, EndoCAM\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862384267433,"sku":"102427","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-102427","provider":"Iright","version":"1.0","type":"link"}