{"product_id":"biolegend-103314","title":"Biolegend, 103314, PerCP\/Cyanine5.5 anti-mouse CD45RB Antibody, 100μg","description":"\u003cp\u003eCD45RB is an isoform of CD45 with exon 5 splicing (encodes B determinant). It is a 220 kD glycoprotein expressed on peripheral B cells, naïve T cells, thymocytes, weakly on macrophages, and dendritic cells. It plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4 + T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Cloned mouse Th2 cell lines\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bottomly K, et al. 1989. Eur. J. Immunol. 19:617. Norian LA and Allen PM. 2004. J. Immunol. 173:835.\u003cbr\u003e\nProduct Citations: Giampaolo S, et al. 2023. iScience. 26:106234. PubMed Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed\u003cbr\u003e\nRRID: AB_1953290 (BioLegend Cat. No. 103313) AB_2284707 (BioLegend Cat. No. 103314)\u003cbr\u003e\nStructure: Phosphatase receptor (CD45 gene exon 5), 220 kD\u003cbr\u003e\nDistribution: Peripheral B cells, naïve T cells, most thymocytes, low density on macrophages and dendritic cells\u003cbr\u003e\nFunction: Phosphatase, T cell development and activation\u003cbr\u003e\nLigand\/Receptor: Galectin-1, CD2, CD3, CD4, Thy1\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, T cells, Thymocytes, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85. 3. Kishihara K, et al. 1993. Cell 74:143. 4. Pulido R, et al. 1988. J. Immunol. 140:3851.\u003cbr\u003e\nGene ID: 19264\u003cbr\u003e\nUniProt: View information about CD45RB on UniProt.org\u003cbr\u003e\nClone: C363-16A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD45RB\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862942208169,"sku":"103314","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-103314","provider":"Iright","version":"1.0","type":"link"}