{"product_id":"biolegend-104307","title":"Biolegend, 104307, PE anti-mouse\/rat CD61 Antibody, 50μg","description":"\u003cp\u003eCD61 is a 110 kD integrin β chain also known as β 3 integrin or gpIIIa. It associates with the integrin α v chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin α IIb chain (CD41) to form the gpIIb\/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of α v \/β 3 integrin or vitronectin receptor to CD31 (PECAM-1).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: Vitronectin receptor protein from the mouse T-cell hybridoma 2B4.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 104325 and 104326).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) Ashkar S, et al. 2000. Science 287:860. (Block) Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) Carlson TR, et al. 2008.135:2193. PubMed Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed\u003cbr\u003e\nProduct Citations: Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed Kang YA, et al. 2023. J Exp Med. 220:. PubMed Huang TC, et al. 2021. Nature. 600:737. PubMed Gawish R, et al. 2022. Elife. 11:. PubMed Zhang J, et al. 2018. Nature. 562:595. PubMed Cordeiro O, et al. 2016. PLoS One. 11: 0151848. PubMed Li H, et al. 2021. Nat Commun. 12:2773. PubMed Wang X, et al. 2021. Cell Res. 31:526. PubMed Aizawa E, et al. 2022. Stem Cell Reports. 17:43. PubMed Bonnardel J, et al. 2019. Immunity. 51:638. PubMed Lin J, et al. 2021. Mol Med. 27:149. PubMed Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed Bleckwehl T, et al. 2021. Nat Commun. 12:5771. PubMed Lyu C, et al. 2022. Cancers (Basel). 14:. PubMed Gao Q, et al. 2021. Stem Cells Transl Med. 10:743. PubMed Manupati K, et al. 2022. Cancers (Basel). 14:. PubMed Zhuo Y, et al. 2018. Oncol Rep. 40:2147. PubMed Yu S, et al. 2022. Nat Commun. 13:2756. PubMed Kinoshita M, et al. 2020. Cell Stem Cell. . PubMed Nasser H, et al. 2020. Cell Death Discov. 0.29375. PubMed Morgani SM, et al. 2021. Biology Open. 10(5):. PubMed Saboya M, et al. 2020. Alcohol Clin Exp Res. 44:831. PubMed Pele T, et al. 2021. Int J Mol Sci. 22:. PubMed Vojtek M, et al. 2021. Life Sci Alliance. 4:. PubMed\u003cbr\u003e\nRRID: AB_313084 (BioLegend Cat. No. 104307) AB_313085 (BioLegend Cat. No. 104308)\u003cbr\u003e\nStructure: Integrin family, 110 kD\u003cbr\u003e\nDistribution: Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells\u003cbr\u003e\nFunction: Attachment to matrix\u003cbr\u003e\nLigand\/Receptor: Fibrinogen, von Willebrand factor, fibronectin\u003cbr\u003e\nCell Type: B cells, Endothelial cells, Macrophages, Mast cells, Megakaryocytes, Monocytes, Osteoclasts, Platelets, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Phillips DR, et al. 1991. Cell. 65:359. 3. Felding-Habermann B, et al. 1993. Curr Opinion Cell Biol. 5:864.\u003cbr\u003e\nGene ID: 1641629302\u003cbr\u003e\nUniProt: View information about CD61 on UniProt.org\u003cbr\u003e\nClone: 2C9.G2 (HMβ3-1)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: β3 integrin, Integrin β3 chain, gpIIIa\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866542919849,"sku":"104307","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-104307","provider":"Iright","version":"1.0","type":"link"}