{"product_id":"biolegend-104705","title":"Biolegend, 104705, FITC anti-mouse CD80 Antibody, 50μg","description":"\u003cp\u003eCD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes\/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nReported Reactivity: Dog\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Armenian Hamster\u003cbr\u003e\nImmunogen: CHO cell line transfected with mouse B7 (CD80)\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104747-104752).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC) Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP) Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block) Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC) Ma XT, et al. 2006. Cancer Res. 66:1169. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) Lawson BR, et al. 2007. J. Immunol. 178:5366. Turnquist HR, et al. 2007. J. Immunol. 178:7018. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed\u003cbr\u003e\nProduct Citations: Akhtar MN, et al. 2022. MBio. 13:e0330921. PubMed Zhuang WR, et al. 2023. Nat Commun. 14:1675. PubMed Do-Thi VA, et al. 2023. Cancer Res Commun. 3:80. PubMed Fu S, et al. 2023. Nat Commun. 14:2248. PubMed Brigo N, et al. 2023. Cells. 12:. PubMed Bhaskar A, et al. 2023. iScience. 26:106644. PubMed Wang D, et al. 2023. Nat Commun. 14:2943. PubMed Rosina M, et al. 2022. Cell Metab. 34:533. PubMed Kawai H, et al. 2021. Asian Pac J Allergy Immunol. :. PubMed Chen C, et al. 2022. Sci Adv. 8:eabo4363. PubMed Chen Y, et al. 2023. Adv Healthc Mater. 12:e2202085. PubMed Wang H, et al. 2022. J Nanobiotechnology. 20:513. PubMed Kimura Y, et al. 2023. Front Immunol. 14:1070196. PubMed Vesin B, et al. 2022. Mol Ther. 30:2984. PubMed Kis-Toth K, et al. 2011. J Immunol. 187:1273. PubMed Jiang Q, et al. 2022. Theranostics. 12:59. PubMed Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed Gravano D, et al. 2011. PLoS One. 6:e13517. PubMed Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed Porciello N, et al. 2018. Nat Commun. 9:1080. PubMed Oyarce C, et al. 2018. Front Immunol. 8:1794. PubMed Liang F, et al. 2021. RSC Adv. 11:35331. PubMed Matsumura F, et al. 2021. J Immunol. 207:938. PubMed Saito S, et al. 2020. Nutrients. 12:. PubMed Sun T, et al. 2018. Front Immunol. 9:1245. PubMed Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed Wang G, et al. 2021. Nat Commun. 12:5733. PubMed Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed Jiang H, et al. 2018. Front Pharmacol. 9:612. PubMed Blank A, et al. 2021. J Pathol. 253:160. PubMed Li X, et al. 2014. PLoS One. 9:108192. PubMed Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed Balashov L 2015. Mult Scler. 21: 1131-1138. PubMed Liang Y, et al. 2014. J Immunol. 192:1277. PubMed Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed Snell LM, et al. 2018. Immunity. 49:678. PubMed Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed Kornstädt L, et al. 2021. Front Immunol. 11:607048. PubMed Qi T, et al. 2020. Cell Rep. 33:108452. PubMed Wang J, et al. 2021. Nat Commun. 12:6198. PubMed Zhou Y, et al. 2020. Front Pharmacol. 10:1579. PubMed Li X, et al. 2009. PLoS One. 4:e6385. PubMed Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed Zhang C, et al. 2022. Nat Commun. 13:3468. PubMed Li Y, et al. 2021. Sci Transl Med. 13:. PubMed Ji W, et al. 2018. Evid Based Complement Alternat Med. 2017:3272649. PubMed Xiong W, et al. 2022. Adv Sci (Weinh). 9:e2103029. PubMed Benhamron S, et al. 2012. PLoS One. 7:e35602. PubMed Shiozawa S, et al. 2022. iScience. 25:103537. PubMed Gómez LA, et al. 2020. Front Microbiol. 11:1586. PubMed Sanchez M et al. 2017. Cell host \u0026amp; microbe. 22(4):543-551 . PubMed Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed Zhou J, et al. 2022. Int J Oncol. 61: . PubMed Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed Xia Y, et al. 2018. Cell. 175:1059. PubMed Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed Tassi I, et al. 2014. J Immunol. 193:4303. 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No. 104706)\u003cbr\u003e\nStructure: Ig superfamily, 60 kD\u003cbr\u003e\nDistribution: Macrophages, activated B cells and T cells, dendritic cells\u003cbr\u003e\nFunction: T cell costimulation\u003cbr\u003e\nLigand\/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)\u003cbr\u003e\nCell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: CD Molecules, Immune Checkpoint Receptors\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Linsley PS, et al. 1991. J. Exp. Med. 174:561. 3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.\u003cbr\u003e\nGene ID: 12519\u003cbr\u003e\nUniProt: View information about CD80 on UniProt.org\u003cbr\u003e\nClone: 16-10A1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B7-1, B7, Ly-53\u003cbr\u003e\nIsotype: Armenian Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860814221481,"sku":"104705","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-104705","provider":"Iright","version":"1.0","type":"link"}