{"product_id":"biolegend-105722","title":"Biolegend, 105722, Pacific Blue™ anti-mouse CD106 Antibody, 100μg","description":"\u003cp\u003eCD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α 4 \/β 1 integrin) and LPAM-1 (α 4 \/β 7 integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse preadipose cell line PA6\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Violet Laser (405 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining2,3,5-7 of acetone-fixed frozen sections, blocking4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation1 , and spacial biology (IBEX)11,12. The Ultra-LEAF™ purified antibody (Endotoxin \u0026lt; 0.01 EU\/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 \u0026amp; 105728).\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. (IP) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ishiyama N, et al. 1998. Pathobiology 66:274. (IHC) Kinashi T, et al. 1994. Blood Cells 20:25. (Block) Baron JL, et al. 1994. J. Clin. Invest. 93:1700. (Block IHC) Buck CA, et al. 1996. Cell Adhes. Commun. 4:69. (IHC) Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) Meunier MC, et al. 2005. Nature Medicine 11:1222. (Block) PubMed Monnier J, et al. 2012. J. Immunol. 189:956. PubMed Motohashi N, et al. 2013. J Cell Sci. 126:2678. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed Wang Y, et al. 2021. Nat Commun. 12:2343. PubMed\u003cbr\u003e\nRRID: AB_2304290 (BioLegend Cat. No. 105722)\u003cbr\u003e\nStructure: Ig superfamily, 47 kD\u003cbr\u003e\nDistribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells\u003cbr\u003e\nFunction: Adhesion\u003cbr\u003e\nLigand\/Receptor: VLA-4 (α4\/β1 integrin) and LPAM-1 (α4\/β7 integrin)\u003cbr\u003e\nCell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168. 3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767. 4. Koni PA, et al. 2001. J. Exp. Med. 193:741.\u003cbr\u003e\nGene ID: 22329\u003cbr\u003e\nUniProt: View information about CD106 on UniProt.org\u003cbr\u003e\nClone: 429 (MVCAM.A)\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: VCAM-1, INCAM-110\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862402879657,"sku":"105722","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/biolegend-105722","provider":"Iright","version":"1.0","type":"link"}